1yji

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{{STRUCTURE_1yji| PDB=1yji | SCENE= }}
{{STRUCTURE_1yji| PDB=1yji | SCENE= }}
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'''RDC-refined Solution NMR structure of reduced putidaredoxin'''
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===RDC-refined Solution NMR structure of reduced putidaredoxin===
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==Overview==
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Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences have been observed previously for Pdx in terms of binding affinities to CYP101, NMR spectral differences, and dynamic properties. To further characterize these differences, structure refinement of both oxidized and reduced Pdx has been carried out using a hybrid approach utilizing paramagnetic distance restraints and NMR orientational restraints in the form of backbone (15)N residual dipolar couplings. Use of these new restraints has improved the structure of oxidized Pdx considerably over the earlier solution NMR structure without RDC restraints, with the new structure now much closer in overall fold to the recently published X-ray crystal structures. We now observe better defined relative orientations of the major secondary structure elements as also of the conformation of the metal binding loop region. Extension of this approach to structure calculation of reduced Pdx has identified structural differences that are primarily localized for residues in the C-terminal interaction domain consisting of the functionally important residue Trp 106 and regions near the metal binding loop in Pdx. These redox-dependent structural differences in Pdx correlate to dynamic changes observed before and may be linked to differences in binding and electron transfer properties between oxidized and reduced Pdx.
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(as it appears on PubMed at http://www.pubmed.gov), where 15966730 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15966730}}
==About this Structure==
==About this Structure==
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1YJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJI OCA].
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1YJI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YJI OCA].
==Reference==
==Reference==
Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings., Jain NU, Tjioe E, Savidor A, Boulie J, Biochemistry. 2005 Jun 28;44(25):9067-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15966730 15966730]
Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings., Jain NU, Tjioe E, Savidor A, Boulie J, Biochemistry. 2005 Jun 28;44(25):9067-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15966730 15966730]
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A refined model for the solution structure of oxidized putidaredoxin., Pochapsky TC, Jain NU, Kuti M, Lyons TA, Heymont J, Biochemistry. 1999 Apr 13;38(15):4681-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10200155 10200155]
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New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)., Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U, Structure. 1998 Mar 15;6(3):269-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9551550 9551550]
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An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas., Pochapsky TC, Ye XM, Ratnaswamy G, Lyons TA, Biochemistry. 1994 May 31;33(21):6424-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8204575 8204575]
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Redox-dependent 1H NMR spectral features and tertiary structural constraints on the C-terminal region of putidaredoxin., Pochapsky TC, Ratnaswamy G, Patera A, Biochemistry. 1994 May 31;33(21):6433-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8204576 8204576]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Redox]]
[[Category: Redox]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:07:35 2008''

Revision as of 12:07, 28 July 2008

Image:1yji.png

Template:STRUCTURE 1yji

RDC-refined Solution NMR structure of reduced putidaredoxin

Template:ABSTRACT PUBMED 15966730

About this Structure

1YJI is a Single protein structure of sequence from Pseudomonas putida. Full experimental information is available from OCA.

Reference

Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings., Jain NU, Tjioe E, Savidor A, Boulie J, Biochemistry. 2005 Jun 28;44(25):9067-78. PMID:15966730

A refined model for the solution structure of oxidized putidaredoxin., Pochapsky TC, Jain NU, Kuti M, Lyons TA, Heymont J, Biochemistry. 1999 Apr 13;38(15):4681-90. PMID:10200155

New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)., Muller A, Muller JJ, Muller YA, Uhlmann H, Bernhardt R, Heinemann U, Structure. 1998 Mar 15;6(3):269-80. PMID:9551550

An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas., Pochapsky TC, Ye XM, Ratnaswamy G, Lyons TA, Biochemistry. 1994 May 31;33(21):6424-32. PMID:8204575

Redox-dependent 1H NMR spectral features and tertiary structural constraints on the C-terminal region of putidaredoxin., Pochapsky TC, Ratnaswamy G, Patera A, Biochemistry. 1994 May 31;33(21):6433-41. PMID:8204576

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