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1w8f

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[[Image:1w8f.gif|left|200px]]
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{{STRUCTURE_1w8f| PDB=1w8f | SCENE= }}
{{STRUCTURE_1w8f| PDB=1w8f | SCENE= }}
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'''PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)'''
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===PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)===
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==Overview==
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One of the mechanisms contributing to the protection by breast-feeding of the newborn against enteric diseases is related to the ability of human milk oligosaccharides to prevent the attachment of pathogenic bacteria to the duodenual epithelium. Indeed, a variety of fucosylated oligosaccharides, specific to human milk, form part of the innate immune system. In the present study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas aeruginosa, by milk oligosaccharides. Two fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M respectively. Thermodynamic studies indicate that these interactions are dominated by enthalpy. The entropy contribution is slightly favourable when binding to fucose and to the highest-affinity ligand, Lewis a. The high-resolution X-ray structures of two complexes of PA-IIL with milk oligosaccharides allow the precise determination of the conformation of a trisaccharide and a pentasaccharide. The different types of interaction between the oligosaccharides and the protein involve not only hydrogen bonding, but also calcium- and water-bridged contacts, allowing a rationalization of the thermodynamic data. This study provides important structural information about compounds that could be of general application in new therapeutic strategies against bacterial infections.
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(as it appears on PubMed at http://www.pubmed.gov), where 15790314 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15790314}}
==About this Structure==
==About this Structure==
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[[Category: Wimmerova, M.]]
[[Category: Wimmerova, M.]]
[[Category: Lectin,pentassacharide,cystic firosis,pseudomonas aeruginosa,infection of lung]]
[[Category: Lectin,pentassacharide,cystic firosis,pseudomonas aeruginosa,infection of lung]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:18:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:18:51 2008''

Revision as of 12:18, 28 July 2008

Image:1w8f.png

Template:STRUCTURE 1w8f

PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)

Template:ABSTRACT PUBMED 15790314

About this Structure

1W8F is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa., Perret S, Sabin C, Dumon C, Pokorna M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerova M, Mitchell EP, Imberty A, Biochem J. 2005 Jul 15;389(Pt 2):325-32. PMID:15790314

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