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| | {{STRUCTURE_3bon| PDB=3bon | SCENE= }} | | {{STRUCTURE_3bon| PDB=3bon | SCENE= }} |
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| - | '''Structure of the C. botulinum neurotoxin serotype A with Zn2+ cofactor bound'''
| + | ===Structure of the C. botulinum neurotoxin serotype A with Zn2+ cofactor bound=== |
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| - | ==Overview==
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| - | The Clostridium botulinum neurotoxin serotype A light chain (BoNT/A-LC) is a Zn(II)-dependent metalloprotease that blocks the release of acetylcholine at the neuromuscular junction by cleaving SNAP-25, one of the SNARE proteins required for exocytosis. Because of the potential for use of the toxin in bioterrorism and the increasingly widespread application of the toxin in the medical field, there is significant interest in the development of small-molecule inhibitors of the metalloprotease. Efforts to design such inhibitors have not benefited from knowledge of how peptides bind to the active site since the enzyme-peptide structures available previously either were not occupied in the vicinity of the catalytic Zn(II) ion or did not represent the product of SNAP-25 substrate cleavage. Herein we report the 1.4 A-resolution X-ray crystal structure of a complex between the BoNT/A-LC and the inhibitory peptide N-Ac-CRATKML, the first structure of the light chain with an inhibitory peptide bound at the catalytic Zn(II) ion. The peptide is bound with the Cys S gamma atom coordinating the metal ion. Surprisingly, the cysteine sulfur is oxidized to the sulfenic acid form. Given the unstable nature of this species in solution, is it likely that oxidation occurs on the enzyme. In addition to the peptide-bound structure, we report two structures of the unliganded light chain with and without the Zn(II) cofactor bound at 1.25 and 1.20 A resolution, respectively. The two structures are nearly identical, confirming that the Zn(II) ion plays a purely catalytic role. Additionally, the structure of the Zn(II)-bound uncomplexed enzyme allows identification of the catalytic water molecule and a second water molecule that occupies the same position as the peptidic oxygen in the tetrahedral intermediate. This observation suggests that the enzyme active site is prearranged to stabilize the tetrahedral intermediate of the protease reaction. | + | The line below this paragraph, {{ABSTRACT_PUBMED_18457419}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18457419 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18457419}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Metalloprotease]] | | [[Category: Metalloprotease]] |
| | [[Category: Neurotoxin]] | | [[Category: Neurotoxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 21:50:10 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:29:06 2008'' |
Revision as of 12:29, 28 July 2008
Template:STRUCTURE 3bon
Structure of the C. botulinum neurotoxin serotype A with Zn2+ cofactor bound
Template:ABSTRACT PUBMED 18457419
About this Structure
3BON is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
Reference
Catalytic features of the botulinum neurotoxin a light chain revealed by high resolution structure of an inhibitory peptide complex., Silvaggi NR, Wilson D, Tzipori S, Allen KN, Biochemistry. 2008 May 27;47(21):5736-45. Epub 2008 May 6. PMID:18457419
Page seeded by OCA on Mon Jul 28 15:29:06 2008
