1xx3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1xx3.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1xx3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1xx3| PDB=1xx3 | SCENE= }}
{{STRUCTURE_1xx3| PDB=1xx3 | SCENE= }}
-
'''Solution Structure of Escherichia coli TonB-CTD'''
+
===Solution Structure of Escherichia coli TonB-CTD===
-
==Overview==
+
<!--
-
The TonB protein transduces energy from the proton gradient across the cytoplasmic membrane of Gram-negative bacteria to TonB-dependent outer membrane receptors. It is a critically important protein in iron uptake, and deletion of this protein is known to decrease virulence of bacteria in animal models. This system has been used for Trojan horse antibiotic delivery. Here, we describe the high-resolution solution structure of Escherichia coli TonB residues 103-239 (TonB-CTD). TonB-CTD is monomeric with an unstructured N terminus (103-151) and a well structured C terminus (152-239). The structure contains a four-stranded antiparallel beta-sheet packed against two alpha-helices and an extended strand in a configuration homologous to the C-terminal domain of the TolA protein. Chemical shift perturbations to the TonB-CTD (1)H-(15)N HSCQ spectrum titrated with TonB box peptides modeled from the E.coli FhuA, FepA and BtuB proteins were all equivalent, indicating that all three peptides bind to the same region of TonB. Isothermal titration calorimetry measurements demonstrate that TonB-CTD interacts with the FhuA-derived peptide with a K(D)=36(+/-7) microM. On the basis of chemical shift data, the position of Gln160, and comparison to the TolA gp3 N1 complex crystal structure, we propose that the TonB box binds to TonB-CTD along the beta3-strand.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15644214}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15644214 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15644214}}
==About this Structure==
==About this Structure==
-
1XX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX3 OCA].
+
1XX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX3 OCA].
==Reference==
==Reference==
Line 30: Line 34:
[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Tonb-ctd]]
[[Category: Tonb-ctd]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:37:00 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:31:55 2008''

Revision as of 12:32, 28 July 2008

Image:1xx3.png

Template:STRUCTURE 1xx3

Solution Structure of Escherichia coli TonB-CTD

Template:ABSTRACT PUBMED 15644214

About this Structure

1XX3 is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

The solution structure of the C-terminal domain of TonB and interaction studies with TonB box peptides., Sean Peacock R, Weljie AM, Peter Howard S, Price FD, Vogel HJ, J Mol Biol. 2005 Feb 4;345(5):1185-97. Epub 2004 Dec 15. PMID:15644214

Page seeded by OCA on Mon Jul 28 15:31:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xx3"
Personal tools