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1onm

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[[Image:1onm.gif|left|200px]]
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{{STRUCTURE_1onm| PDB=1onm | SCENE= }}
{{STRUCTURE_1onm| PDB=1onm | SCENE= }}
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'''Solution Structure of a DNA duplex containing A:G mismatch. d(GCTTCAGTCGT):d(ACGACGGAAGC)'''
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===Solution Structure of a DNA duplex containing A:G mismatch. d(GCTTCAGTCGT):d(ACGACGGAAGC)===
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==Overview==
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The efficiency of DNA glycosylases to initiate base excision repair (BER) has been demonstrated to be modulated by the precise sequence context in which the lesion or mismatch is located. In the case of DNA containing an A/G mismatch, in which the recognition and excision of adenine from the mismatch is mediated by the Escherichia coli MutY enzyme, not only does the local sequence context affect the strength of base stacking interactions, but it also modulates the syn/anti conformation around the glycosyl bond of the bases in the mispair. Utilizing prior NMR data to identify DNA sequence contexts that adopt either an anti/anti or a syn/anti configuration at an A/G mismatch, we tested the hypothesis that the initial equilibrium of the mismatched base orientations would modulate the overall efficiency of glycosyl bond scission. By systematically varying the sequence context around a central A/G mismatch within a 30-mer duplex DNA, significant kinetic differences were observed that were consistent with this hypothesis. Since the relative efficiency of the kinetics fell into only two groupings, a NMR study was conducted on a DNA sequence context of unknown syn/anti conformation. These data established that the relative syn/anti conformation did not correlate with the excision efficiency, as well as there being a lack of correlation between kinetics and thermal stability of these DNAs.
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(as it appears on PubMed at http://www.pubmed.gov), where 12893083 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12893083}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONM OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONM OCA].
==Reference==
==Reference==
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[[Category: A:g mismatch]]
[[Category: A:g mismatch]]
[[Category: A:g mispair]]
[[Category: A:g mispair]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:04:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:34:47 2008''

Revision as of 12:34, 28 July 2008

Image:1onm.png

Template:STRUCTURE 1onm

Solution Structure of a DNA duplex containing A:G mismatch. d(GCTTCAGTCGT):d(ACGACGGAAGC)

Template:ABSTRACT PUBMED 12893083

About this Structure

Full experimental information is available from OCA.

Reference

Initiation of repair of A/G mismatches is modulated by sequence context., Sanchez AM, Volk DE, Gorenstein DG, Lloyd RS, DNA Repair (Amst). 2003 Aug 12;2(8):863-78. PMID:12893083

Page seeded by OCA on Mon Jul 28 15:34:47 2008

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