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2g8g

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{{STRUCTURE_2g8g| PDB=2g8g | SCENE= }}
{{STRUCTURE_2g8g| PDB=2g8g | SCENE= }}
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'''Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4'''
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===Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4===
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==Overview==
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The adeno-associated viruses (AAVs) can package and deliver foreign DNA into cells for corrective gene delivery applications. The AAV serotypes have distinct cell binding, transduction, and antigenic characteristics that have been shown to be dictated by the capsid viral protein (VP) sequence. To understand the contribution of capsid structure to these properties, we have determined the crystal structure of AAV serotype 4 (AAV4), one of the most diverse serotypes with respect to capsid protein sequence and antigenic reactivity. Structural comparison of AAV4 to AAV2 shows conservation of the core beta strands (betaB to betaI) and helical (alphaA) secondary structure elements, which also exist in all other known parvovirus structures. However, surface loop variations (I to IX), some containing compensating structural insertions and deletions in adjacent regions, result in local topological differences on the capsid surface. These include AAV4 having a deeper twofold depression, wider and rounder protrusions surrounding the threefold axes, and a different topology at the top of the fivefold channel from that of AAV2. Also, the previously observed "valleys" between the threefold protrusions, containing AAV2's heparin binding residues, are narrower in AAV4. The observed differences in loop topologies at subunit interfaces are consistent with the inability of AAV2 and AAV4 VPs to combine for mosaic capsid formation in efforts to engineer novel tropisms. Significantly, all of the surface loop variations are associated with amino acids reported to affect receptor recognition, transduction, and anticapsid antibody reactivity for AAV2. This observation suggests that these capsid regions may also play similar roles in the other AAV serotypes.
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(as it appears on PubMed at http://www.pubmed.gov), where 16971437 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16971437}}
==About this Structure==
==About this Structure==
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[[Category: Gene therapy]]
[[Category: Gene therapy]]
[[Category: Icosahedral virus]]
[[Category: Icosahedral virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:49:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:36:57 2008''

Revision as of 12:37, 28 July 2008

Image:2g8g.png

Template:STRUCTURE 2g8g

Structurally mapping the diverse phenotype of Adeno-Associated Virus serotype 4

Template:ABSTRACT PUBMED 16971437

About this Structure

2G8G is a Single protein structure of sequence from Viruses. Full crystallographic information is available from OCA.

Reference

Structurally mapping the diverse phenotype of adeno-associated virus serotype 4., Govindasamy L, Padron E, McKenna R, Muzyczka N, Kaludov N, Chiorini JA, Agbandje-McKenna M, J Virol. 2006 Dec;80(23):11556-70. Epub 2006 Sep 13. PMID:16971437

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