1xge

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{{STRUCTURE_1xge| PDB=1xge | SCENE= }}
{{STRUCTURE_1xge| PDB=1xge | SCENE= }}
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'''Dihydroorotase from Escherichia coli: Loop Movement and Cooperativity between subunits'''
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===Dihydroorotase from Escherichia coli: Loop Movement and Cooperativity between subunits===
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==Overview==
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Escherichia coli dihydroorotase has been crystallized in the presence of the product, L-dihydroorotate (L-DHO), and the structure refined at 1.9A resolution. The structure confirms that previously reported (PDB entry 1J79), crystallized in the presence of the substrate N-carbamyl-D,L-aspartate (D, L-CA-asp), which had a dimer in the asymmetric unit, with one subunit having the substrate, L-CA-asp bound at the active site and the other having L-DHO. Importantly, no explanation for the unusual structure was given. Our results now show that a loop comprised of residues 105-115 has different conformations in the two subunits. In the case of the L-CA-asp-bound subunit, this loop reaches in toward the active site and makes hydrogen-bonding contact with the bound substrate molecule. For the L-DHO-bound subunit, the loop faces in the opposite direction and forms part of the surface of the protein. Analysis of the kinetics for conversion of L-DHO to L-CA-asp at low concentrations of L-DHO shows positive cooperativity with a Hill coefficient n=1.57(+/-0.13). Communication between subunits in the dimer may occur via cooperative conformational changes of the side-chains of a tripeptide from each subunit: Arg256-His257-Arg258, near the subunit interface.
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(as it appears on PubMed at http://www.pubmed.gov), where 15826651 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15826651}}
==About this Structure==
==About this Structure==
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[[Category: Maher, M J.]]
[[Category: Maher, M J.]]
[[Category: Tim barrel]]
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Revision as of 12:46, 28 July 2008

Image:1xge.png

Template:STRUCTURE 1xge

Dihydroorotase from Escherichia coli: Loop Movement and Cooperativity between subunits

Template:ABSTRACT PUBMED 15826651

About this Structure

1XGE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits., Lee M, Chan CW, Mitchell Guss J, Christopherson RI, Maher MJ, J Mol Biol. 2005 May 6;348(3):523-33. PMID:15826651

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