2iaw

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[[Image:2iaw.gif|left|200px]]
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{{STRUCTURE_2iaw| PDB=2iaw | SCENE= }}
{{STRUCTURE_2iaw| PDB=2iaw | SCENE= }}
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'''Crystal structure of squid ganglion DFPase N175D mutant'''
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===Crystal structure of squid ganglion DFPase N175D mutant===
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==Overview==
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The active site, the substrate binding site, and the metal binding sites of the diisopropylfluorophosphatase (DFPase) from Loligo vulgaris have been modified by means of site-directed mutagenesis to improve our understanding of the reaction mechanism. Enzymatic characterization of mutants located in the major groove of the substrate binding pocket indicates that large hydrophobic side chains at these positions are favorable for substrate turnover. Moreover, the active site residue His287 proved to be beneficial, but not essential, for DFP hydrolysis. In most cases, hydrophobic side chains at position 287 led to significant catalytic activities although reduced relative to the wild-type enzyme. With respect to the Ca-1 binding site, where catalysis occurs, various mutants indicated that the net charge at this calcium-binding site as well as the relative positions of the charged calcium ligands is crucial for catalytic activity. The importance of the electrostatic potential at the active site was furthermore revealed by various mutations of residues lining the interior of the central water-filled tunnel, which traverses the entire protein structure. In this respect, the structural features of residue His181, which is located at the opposite end of the DFPase tunnel relative to the active site, were characterized extensively. It was concluded that a tunnel-spanning hydrogen bond network, which includes a large number of apparently slow exchanging water molecules, relays any modifications in the electrostatics of the system to the active site, thus affecting the catalytic reactivity of the enzyme.
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The line below this paragraph, {{ABSTRACT_PUBMED_15966726}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15966726 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15966726}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme., Katsemi V, Lucke C, Koepke J, Lohr F, Maurer S, Fritzsch G, Ruterjans H, Biochemistry. 2005 Jun 28;44(25):9022-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15966726 15966726]
Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme., Katsemi V, Lucke C, Koepke J, Lohr F, Maurer S, Fritzsch G, Ruterjans H, Biochemistry. 2005 Jun 28;44(25):9022-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15966726 15966726]
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Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris., Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H, Structure. 2001 Jun;9(6):493-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11435114 11435114]
[[Category: Diisopropyl-fluorophosphatase]]
[[Category: Diisopropyl-fluorophosphatase]]
[[Category: Loligo vulgaris]]
[[Category: Loligo vulgaris]]
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[[Category: Calcium-binding site]]
[[Category: Calcium-binding site]]
[[Category: Phosphotriesterase]]
[[Category: Phosphotriesterase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:16:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:46:13 2008''

Revision as of 12:46, 28 July 2008

Image:2iaw.png

Template:STRUCTURE 2iaw

Crystal structure of squid ganglion DFPase N175D mutant

Template:ABSTRACT PUBMED 15966726

About this Structure

2IAW is a Single protein structure of sequence from Loligo vulgaris. Full crystallographic information is available from OCA.

Reference

Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme., Katsemi V, Lucke C, Koepke J, Lohr F, Maurer S, Fritzsch G, Ruterjans H, Biochemistry. 2005 Jun 28;44(25):9022-33. PMID:15966726

Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris., Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H, Structure. 2001 Jun;9(6):493-502. PMID:11435114

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