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1za6

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{{STRUCTURE_1za6| PDB=1za6 | SCENE= }}
{{STRUCTURE_1za6| PDB=1za6 | SCENE= }}
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'''The structure of an antitumor CH2-domain-deleted humanized antibody'''
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===The structure of an antitumor CH2-domain-deleted humanized antibody===
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==Overview==
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C(H)2-domain-deleted CC49 (HuCC49DeltaCH2), a recombinant humanized antibody that recognizes the TAG-72 antigen expressed on a variety of human carcinomas, is secreted from cultured cells as a mixture of two homodimeric isoforms. Isoform A contains two covalent interchain disulfide bonds at heavy chain positions 239 and 242, while isoform B fails to develop any interchain disulfide bonds but has 239-242 intrachain disulfide bonds instead. Form A is currently in preclinical development as a therapeutic agent for treating colorectal carcinoma, though form B shows equal efficacy. HuCC49DeltaCH2 form B can be crystallized from sodium formate only in the presence of detergents. X-ray diffraction data were collected on a single cryo-cooled crystal grown with Triton X-100 and the structure was solved by molecular replacement. The model has refined to R=0.246 (R(free)=0.297) for 2.8A data. The antibodies pack in the crystal around crystallographic 2-fold axes as tetramers with approximate 222 symmetry. Atomic force microscopy studies show that this tetrameric structure is the crystal building block and also exists free in the mother liquor. The tetramer is composed of two rings, back-to-back, with a thickness of approximately 83A. Each ring is composed of two antibodies with the complementarity-determining regions (CDR) of the two Fabs of one antibody interacting with the CDR regions of the second antibody in a head-to-head fashion. These rings are approximately 167A long and 112A wide. The C(H)3 domain is inverted with respect to the Fabs when compared to the usual orientation found in conventional antibodies. The polypeptides joining the C(H)3 domains to the Fab portions of the antibody are not seen and are almost certainly disordered. The antigen combining site of HuCC49DeltaCH2 is very similar, but not identical, in topology and charge distribution to that of antibody B72.3, which binds a similar epitope on TAG-72. The combining site consists of a deep cleft, heavily lined with aromatic amino acid side-chains but bounded by numerous charged groups.
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{{ABSTRACT_PUBMED_15854653}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The structure of an antitumor C(H)2-domain-deleted humanized antibody., Larson SB, Day JS, Glaser S, Braslawsky G, McPherson A, J Mol Biol. 2005 May 20;348(5):1177-90. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15854653 15854653]
The structure of an antitumor C(H)2-domain-deleted humanized antibody., Larson SB, Day JS, Glaser S, Braslawsky G, McPherson A, J Mol Biol. 2005 May 20;348(5):1177-90. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15854653 15854653]
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Combined use of AFM and X-ray diffraction to analyze crystals of an engineered, domain-deleted antibody., Larson SB, Kuznetsov YG, Day J, Zhou J, Glaser S, Braslawsky G, McPherson A, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):416-22. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805596 15805596]
[[Category: Braslawsky, G.]]
[[Category: Braslawsky, G.]]
[[Category: Day, J S.]]
[[Category: Day, J S.]]
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[[Category: Ch2-domain-deletion]]
[[Category: Ch2-domain-deletion]]
[[Category: Immunoglobulin fold]]
[[Category: Immunoglobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:22:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:49:12 2008''

Revision as of 12:49, 28 July 2008

Image:1za6.png

Template:STRUCTURE 1za6

The structure of an antitumor CH2-domain-deleted humanized antibody

Template:ABSTRACT PUBMED 15854653

About this Structure

Full crystallographic information is available from OCA.

Reference

The structure of an antitumor C(H)2-domain-deleted humanized antibody., Larson SB, Day JS, Glaser S, Braslawsky G, McPherson A, J Mol Biol. 2005 May 20;348(5):1177-90. Epub 2005 Apr 1. PMID:15854653

Combined use of AFM and X-ray diffraction to analyze crystals of an engineered, domain-deleted antibody., Larson SB, Kuznetsov YG, Day J, Zhou J, Glaser S, Braslawsky G, McPherson A, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):416-22. Epub 2005, Mar 24. PMID:15805596

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