2ggd

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[[Image:2ggd.gif|left|200px]]
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{{STRUCTURE_2ggd| PDB=2ggd | SCENE= }}
{{STRUCTURE_2ggd| PDB=2ggd | SCENE= }}
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'''CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate'''
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===CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate===
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==Overview==
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The engineering of transgenic crops resistant to the broad-spectrum herbicide glyphosate has greatly improved agricultural efficiency worldwide. Glyphosate-based herbicides, such as Roundup, target the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, the functionality of which is absolutely required for the survival of plants. Roundup Ready plants carry the gene coding for a glyphosate-insensitive form of this enzyme, obtained from Agrobacterium sp. strain CP4. Once incorporated into the plant genome, the gene product, CP4 EPSP synthase, confers crop resistance to glyphosate. Although widely used, the molecular basis for this glyphosate-resistance has remained obscure. We generated a synthetic gene coding for CP4 EPSP synthase and characterized the enzyme using kinetics and crystallography. The CP4 enzyme has unexpected kinetic and structural properties that render it unique among the known EPSP synthases. Glyphosate binds to the CP4 EPSP synthase in a condensed, noninhibitory conformation. Glyphosate sensitivity can be restored through a single-site mutation in the active site (Ala-100-Gly), allowing glyphosate to bind in its extended, inhibitory conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 16916934 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16916934}}
==About this Structure==
==About this Structure==
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[[Category: Inside-out alpha/beta barrel]]
[[Category: Inside-out alpha/beta barrel]]
[[Category: Two domain structure]]
[[Category: Two domain structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:04:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:03:48 2008''

Revision as of 13:03, 28 July 2008

Image:2ggd.png

Template:STRUCTURE 2ggd

CP4 EPSP synthase Ala100Gly liganded with S3P and Glyphosate

Template:ABSTRACT PUBMED 16916934

About this Structure

2GGD is a Single protein structure of sequence from Agrobacterium sp.. Full crystallographic information is available from OCA.

Reference

Molecular basis for the herbicide resistance of Roundup Ready crops., Funke T, Han H, Healy-Fried ML, Fischer M, Schonbrunn E, Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13010-5. Epub 2006 Aug 17. PMID:16916934

Page seeded by OCA on Mon Jul 28 16:03:48 2008

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