3c25

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{{STRUCTURE_3c25| PDB=3c25 | SCENE= }}
{{STRUCTURE_3c25| PDB=3c25 | SCENE= }}
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'''Crystal Structure of NotI Restriction Endonuclease Bound to Cognate DNA'''
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===Crystal Structure of NotI Restriction Endonuclease Bound to Cognate DNA===
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==Overview==
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The structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5'-GCGGCCGC-3', has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and to map DNA methylation status. NotI contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral Cys4 motif. This domain positions nearby protein elements for DNA recognition, and serves a structural role. While recognition of the central six base pairs of the target is accomplished via a saturated hydrogen bond network typical of restriction enzymes, the most peripheral base pairs are engaged in a single direct contact in the major groove, reflecting reduced pressure to recognize those positions. NotI may represent an evolutionary intermediate between mobile endonucleases (which recognize longer target sites) and canonical restriction endonucleases.
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{{ABSTRACT_PUBMED_18400177}}
==About this Structure==
==About this Structure==
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[[Category: Restriction endonuclease]]
[[Category: Restriction endonuclease]]
[[Category: Restriction enzyme fold]]
[[Category: Restriction enzyme fold]]
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Revision as of 13:06, 28 July 2008

Image:3c25.png

Template:STRUCTURE 3c25

Crystal Structure of NotI Restriction Endonuclease Bound to Cognate DNA

Template:ABSTRACT PUBMED 18400177

About this Structure

3C25 is a Protein complex structure of sequences from Nocardia otitidiscaviarum. This structure supersedes the now removed PDB entry 3bvr. Full crystallographic information is available from OCA.

Reference

Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding., Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL, Structure. 2008 Apr;16(4):558-69. PMID:18400177

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