1n9g
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(New page: 200px<br /><applet load="1n9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n9g, resolution 1.98Å" /> '''Mitochondrial 2-enoy...)
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Revision as of 19:57, 20 November 2007
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Mitochondrial 2-enoyl thioester reductase Etr1p/Etr2p heterodimer from Candida tropicalis
Overview
Here we report on the cloning of a Candida tropicalis gene, ETR2, that is, closely related to ETR1. Both genes encode enzymatically active 2-enoyl, thioester reductases involved in mitochondrial synthesis of fatty acids, (fatty acid synthesis type II) and respiratory competence. The 5'- and, 3'-flanking (coding) regions of ETR2 and ETR1 are about 90% (97%), identical, indicating that the genes have evolved via gene duplication., The gene products differ in three amino acid residues: Ile67 (Val), Ala92, (Thr), and Lys251 (Arg) in Etr2p (Etr1p). Quantitative PCR analysis and, reverse transcriptase-PCR indicated that both genes were expressed about, equally in fermenting and ETR1 predominantly respiring yeast cells. Like, the situation with ETR1, expression of ETR2 in respiration-deficient, Saccharomyces cerevisiae mutant cells devoid of Ybr026p/Etr1p was able to, restore growth on glycerol. Triclosan that is used as an antibacterial, agent against fatty acid synthesis type II 2-enoyl thioester reductases, inhibited growth of FabI overexpressing mutant yeast cells but was not, able to inhibit respiratory growth of the ETR2- or ETR1-complemented, mutant yeast cells. Resolving of crystal structures obtained via Etr2p and, Etr1p co-crystallization indicated that all possible dimer variants occur, in the same asymmetric unit, suggesting that similar dimer formation also, takes place in vivo.
About this Structure
1N9G is a Protein complex structure of sequences from Candida tropicalis with SO4 and NAP as ligands. Full crystallographic information is available from OCA.
Reference
Candida tropicalis expresses two mitochondrial 2-enoyl thioester reductases that are able to form both homodimers and heterodimers., Torkko JM, Koivuranta KT, Kastaniotis AJ, Airenne TT, Glumoff T, Ilves M, Hartig A, Gurvitz A, Hiltunen JK, J Biol Chem. 2003 Oct 17;278(42):41213-20. Epub 2003 Jul 30. PMID:12890667
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