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1y44

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[[Image:1y44.gif|left|200px]]
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{{STRUCTURE_1y44| PDB=1y44 | SCENE= }}
{{STRUCTURE_1y44| PDB=1y44 | SCENE= }}
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'''Crystal structure of RNase Z'''
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===Crystal structure of RNase Z===
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==Overview==
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Transfer RNAs (tRNAs) are synthesized as part of longer primary transcripts that require processing of both their 3' and 5' extremities in every living organism known. The 5' side is processed (matured) by the ubiquitously conserved endonucleolytic ribozyme, RNase P, whereas removal of the 3' tails can be either exonucleolytic or endonucleolytic. The endonucleolytic pathway is catalysed by an enzyme known as RNase Z, or 3' tRNase. RNase Z cleaves precursor tRNAs immediately after the discriminator base (the unpaired nucleotide 3' to the last base pair of the acceptor stem, used as an identity determinant by many aminoacyl-tRNA synthetases) in most cases, yielding a tRNA primed for addition of the CCA motif by nucleotidyl transferase. Here we report the crystal structure of Bacillus subtilis RNase Z at 2.1 A resolution, and propose a mechanism for tRNA recognition and cleavage. The structure explains the allosteric properties of the enzyme, and also sheds light on the mechanisms of inhibition by the CCA motif and long 5' extensions. Finally, it highlights the extraordinary adaptability of the metallo-hydrolase domain of the beta-lactamase family for the hydrolysis of covalent bonds.
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The line below this paragraph, {{ABSTRACT_PUBMED_15654328}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15654328 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15654328}}
==About this Structure==
==About this Structure==
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[[Category: Sierra-Gallay, I L.de la.]]
[[Category: Sierra-Gallay, I L.de la.]]
[[Category: Zinc-dependent metal hydrolase]]
[[Category: Zinc-dependent metal hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:51:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:18:33 2008''

Revision as of 13:18, 28 July 2008

Image:1y44.png

Template:STRUCTURE 1y44

Crystal structure of RNase Z

Template:ABSTRACT PUBMED 15654328

About this Structure

1Y44 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z., de la Sierra-Gallay IL, Pellegrini O, Condon C, Nature. 2005 Feb 10;433(7026):657-61. Epub 2005 Jan 16. PMID:15654328

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