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1igm

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[[Image:1igm.gif|left|200px]]
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{{STRUCTURE_1igm| PDB=1igm | SCENE= }}
{{STRUCTURE_1igm| PDB=1igm | SCENE= }}
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'''THREE DIMENSIONAL STRUCTURE OF AN FV FROM A HUMAN IGM IMMUNOGLOBULIN'''
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===THREE DIMENSIONAL STRUCTURE OF AN FV FROM A HUMAN IGM IMMUNOGLOBULIN===
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==Overview==
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An IgM(kappa) immunoglobulin from a patient (Pot) with Waldenstrom's macroglobulinemia was hydrolyzed with pepsin to release a fragment consisting of the 'variable' (V) domains of the light and heavy chains plus eight residue 'tails' from the 'constant' (C) domains. The crystal structure of this fragment was determined at 2.3 A resolution by molecular replacement and crystallographic refinement methods. When examined separately, the light chain component closely resembles another human kappa chain (Rei) in both the beta-pleated sheet regions and the 'hypervariable' loops. The conserved pleated sheets in the heavy chain are similar to those in the human Kol IgG1 protein, but the third hypervariable loop in particular is different from that in any immunoglobulin structure described to date. As in the Kol protein, this loop blocks the access to any internal active site along the light-heavy chain interface. Unlike the Kol protein, however, the loop does not protrude beyond the boundaries of a conventional antigen combining site. Instead, it forms a very compact structure, which fills almost all residual space between the domains. This is an example of one dominant complementarity-determining region (CDR) essentially negating the diversity possible with five other CDRs in the two chains. Ordered water molecules are associated with light chain constituents along the interface, but not with CDR3 of the heavy chain. In screening exercises the Pot IgM failed to bind a wide variety of peptides. Together, the results suggest that ligand binding can only occur on external surfaces of the protein. These surfaces carry a limited number of side chains usually assigned to CDRs in more typical antibodies.
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The line below this paragraph, {{ABSTRACT_PUBMED_1447781}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 1447781 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1447781}}
==About this Structure==
==About this Structure==
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[[Category: Guddat, L W.]]
[[Category: Guddat, L W.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:58:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:26:58 2008''

Revision as of 13:27, 28 July 2008

Image:1igm.png

Template:STRUCTURE 1igm

THREE DIMENSIONAL STRUCTURE OF AN FV FROM A HUMAN IGM IMMUNOGLOBULIN

Template:ABSTRACT PUBMED 1447781

About this Structure

1IGM is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of an Fv from a human IgM immunoglobulin., Fan ZC, Shan L, Guddat LW, He XM, Gray WR, Raison RL, Edmundson AB, J Mol Biol. 1992 Nov 5;228(1):188-207. PMID:1447781

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