1zsz

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[[Image:1zsz.gif|left|200px]]
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{{Seed}}
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[[Image:1zsz.png|left|200px]]
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{{STRUCTURE_1zsz| PDB=1zsz | SCENE= }}
{{STRUCTURE_1zsz| PDB=1zsz | SCENE= }}
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'''Crystal structure of a computationally designed SspB heterodimer'''
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===Crystal structure of a computationally designed SspB heterodimer===
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==Overview==
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Protein-protein interactions can be designed computationally by using positive strategies that maximize the stability of the desired structure and/or by negative strategies that seek to destabilize competing states. Here, we compare the efficacy of these methods in reengineering a protein homodimer into a heterodimer. The stability-design protein (positive design only) was experimentally more stable than the specificity-design heterodimer (positive and negative design). By contrast, only the specificity-design protein assembled as a homogenous heterodimer in solution, whereas the stability-design protein formed a mixture of homodimer and heterodimer species. The experimental stabilities of the engineered proteins correlated roughly with their calculated stabilities, and the crystal structure of the specificity-design heterodimer showed most of the predicted side-chain packing interactions and a main-chain conformation indistinguishable from the wild-type structure. These results indicate that the design simulations capture important features of both stability and structure and demonstrate that negative design can be critical for attaining specificity when competing states are close in structure space.
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The line below this paragraph, {{ABSTRACT_PUBMED_16129838}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16129838 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16129838}}
==About this Structure==
==About this Structure==
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[[Category: Protein design]]
[[Category: Protein design]]
[[Category: Specificity]]
[[Category: Specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:02:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:28:35 2008''

Revision as of 13:28, 28 July 2008

Image:1zsz.png

Template:STRUCTURE 1zsz

Crystal structure of a computationally designed SspB heterodimer

Template:ABSTRACT PUBMED 16129838

About this Structure

1ZSZ is a Protein complex structure of sequences from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

Specificity versus stability in computational protein design., Bolon DN, Grant RA, Baker TA, Sauer RT, Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12724-9. Epub 2005 Aug 29. PMID:16129838

Page seeded by OCA on Mon Jul 28 16:28:35 2008

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