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| - | [[Image:2hi5.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2hi5.png|left|200px]] |
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| | {{STRUCTURE_2hi5| PDB=2hi5 | SCENE= }} | | {{STRUCTURE_2hi5| PDB=2hi5 | SCENE= }} |
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| - | '''Model for bacteriophage fd from cryo-EM'''
| + | ===Model for bacteriophage fd from cryo-EM=== |
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| - | ==Overview==
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| - | Many thin helical polymers, including bacterial pili and filamentous bacteriophage, have been seen as refractory to high-resolution studies by electron microscopy. Studies of the quaternary structure of such filaments have depended upon techniques such as modeling or X-ray fiber diffraction, given that direct visualization of the subunit organization has not been possible. We report the first image reconstruction of a filamentous virus, bacteriophage fd, by cryoelectron microscopy. Although these thin ( approximately 70 A in diameter) rather featureless filaments scatter weakly, we have been able to achieve a nominal resolution of approximately 8 A using an iterative helical reconstruction procedure. We show that two different conformations of the virus exist, and that in both states the subunits are packed differently than in conflicting models previously proposed on the basis of X-ray fiber diffraction or solid-state NMR studies. A significant fraction of the population of wild-type fd is either disordered or in multiple conformational states, while in the presence of the Y21M mutation, this heterogeneity is greatly reduced, consistent with previous observations. These results show that new computational approaches to helical reconstruction can greatly extend the ability to visualize heterogeneous protein polymers at a reasonably high resolution.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16843489}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16843489 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16843489}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Helix]] | | [[Category: Helix]] |
| | [[Category: Structural protein]] | | [[Category: Structural protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:19:23 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:32:07 2008'' |
Revision as of 13:32, 28 July 2008
Template:STRUCTURE 2hi5
Model for bacteriophage fd from cryo-EM
Template:ABSTRACT PUBMED 16843489
About this Structure
2HI5 is a Single protein structure of sequence from Enterobacteria phage fd. Full crystallographic information is available from OCA.
Reference
The structure of a filamentous bacteriophage., Wang YA, Yu X, Overman S, Tsuboi M, Thomas GJ Jr, Egelman EH, J Mol Biol. 2006 Aug 11;361(2):209-15. Epub 2006 Jun 30. PMID:16843489
Page seeded by OCA on Mon Jul 28 16:32:07 2008
