1y5o

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{{STRUCTURE_1y5o| PDB=1y5o | SCENE= }}
{{STRUCTURE_1y5o| PDB=1y5o | SCENE= }}
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'''NMR structure of the amino-terminal domain from the Tfb1 subunit of yeast TFIIH'''
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===NMR structure of the amino-terminal domain from the Tfb1 subunit of yeast TFIIH===
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==Overview==
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General transcription factor IIH (TFIIH) is recruited to the preinitiation complex (PIC) through direct interactions between its p62 (Tfb1) subunit and the carboxyl-terminal domain of TFIIEalpha. TFIIH has also been shown to interact with a number of transcriptional activator proteins through interactions with the same p62 (Tfb1) subunit. We have determined the NMR solution structure of the amino-terminal domain from the Tfb1 subunit of yeast TFIIH (Tfb1(1-115)). Like the corresponding domain from the human p62 protein, Tfb1(1-115) contains a PH domain fold despite a low level of sequence identity between the two functionally homologous proteins. In addition, we have performed in vitro binding studies that demonstrate that the PH domains of Tfb1 and p62 specifically bind to monophosphorylated inositides [PtdIns(5)P and PtdIns(3)P]. NMR chemical shift mapping demonstrated that the PtdIns(5)P binding site on Tfb1 (p62) is located in the basic pocket formed by beta-strands beta5-beta7 of the PH domain fold. Interestingly, the structural composition of the PtdIns(5)P binding site is different from the composition of the binding sites for phosphoinositides on prototypic PH domains. We have also determined that the PH domains from Tfb1 and p62 are sufficient for binding to the activation domain of VP16. NMR chemical shift mapping demonstrated that the VP16 binding site within the PH domain of Tfb1 (p62) overlaps with the PtdIns(5)P binding site on Tfb1 (p62). These results provide new information about the recognition of phosphoinositides by PH domains, and point to a potential role for phosphoinositides in VP16 regulation.
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{{ABSTRACT_PUBMED_15909982}}
==About this Structure==
==About this Structure==
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1Y5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y5O OCA].
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1Y5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y5O OCA].
==Reference==
==Reference==
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[[Category: Tfiih]]
[[Category: Tfiih]]
[[Category: Vp16]]
[[Category: Vp16]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:54:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:49:18 2008''

Revision as of 13:49, 28 July 2008

Image:1y5o.png

Template:STRUCTURE 1y5o

NMR structure of the amino-terminal domain from the Tfb1 subunit of yeast TFIIH

Template:ABSTRACT PUBMED 15909982

About this Structure

1Y5O is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

NMR structure of the amino-terminal domain from the Tfb1 subunit of TFIIH and characterization of its phosphoinositide and VP16 binding sites., Di Lello P, Nguyen BD, Jones TN, Potempa K, Kobor MS, Legault P, Omichinski JG, Biochemistry. 2005 May 31;44(21):7678-86. PMID:15909982

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