2fco

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{{STRUCTURE_2fco| PDB=2fco | SCENE= }}
{{STRUCTURE_2fco| PDB=2fco | SCENE= }}
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'''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase'''
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===Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase===
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==Overview==
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Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.
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(as it appears on PubMed at http://www.pubmed.gov), where 17557334 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17557334}}
==About this Structure==
==About this Structure==
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[[Category: Flexibility]]
[[Category: Flexibility]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:44:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:00:41 2008''

Revision as of 14:00, 28 July 2008

Image:2fco.png

Template:STRUCTURE 2fco

Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase

Template:ABSTRACT PUBMED 17557334

About this Structure

2FCO is a Single protein structure of sequence from Geobacillus kaustophilus. Full crystallographic information is available from OCA.

Reference

Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:17557334

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