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| - | [[Image:2f1z.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2f1z| PDB=2f1z | SCENE= }} | | {{STRUCTURE_2f1z| PDB=2f1z | SCENE= }} |
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| - | '''Crystal structure of HAUSP'''
| + | ===Crystal structure of HAUSP=== |
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| - | ==Overview==
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| - | Herpesvirus-associated ubiquitin-specific protease (HAUSP, also known as USP7), a deubiquitylating enzyme of the ubiquitin-specific processing protease family, specifically deubiquitylates both p53 and MDM2, hence playing an important yet enigmatic role in the p53-MDM2 pathway. Here we demonstrate that both p53 and MDM2 specifically recognize the N-terminal tumor necrosis factor-receptor associated factor (TRAF)-like domain of HAUSP in a mutually exclusive manner. HAUSP preferentially forms a stable HAUSP-MDM2 complex even in the presence of excess p53. The HAUSP-binding elements were mapped to a peptide fragment in the carboxy-terminus of p53 and to a short-peptide region preceding the acidic domain of MDM2. The crystal structures of the HAUSP TRAF-like domain in complex with p53 and MDM2 peptides, determined at 2.3-A and 1.7-A resolutions, respectively, reveal that the MDM2 peptide recognizes the same surface groove in HAUSP as that recognized by p53 but mediates more extensive interactions. Structural comparison led to the identification of a consensus peptide-recognition sequence by HAUSP. These results, together with the structure of a combined substrate-binding-and-deubiquitylation domain of HAUSP, provide important insights into regulation of the p53-MDM2 pathway by HAUSP.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16402859}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16402859 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16402859}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ubp]] | | [[Category: Ubp]] |
| | [[Category: Usp7]] | | [[Category: Usp7]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:22:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:00:56 2008'' |
Revision as of 14:01, 28 July 2008
Template:STRUCTURE 2f1z
Crystal structure of HAUSP
Template:ABSTRACT PUBMED 16402859
About this Structure
2F1Z is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of competitive recognition of p53 and MDM2 by HAUSP/USP7: implications for the regulation of the p53-MDM2 pathway., Hu M, Gu L, Li M, Jeffrey PD, Gu W, Shi Y, PLoS Biol. 2006 Feb;4(2):e27. Epub 2006 Jan 17. PMID:16402859
Page seeded by OCA on Mon Jul 28 17:00:56 2008
