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2hoo

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{{STRUCTURE_2hoo| PDB=2hoo | SCENE= }}
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'''Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine'''
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===Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine===
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==Overview==
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Riboswitches are noncoding mRNA elements that bind small-molecule metabolites with high affinity and specificity, and they regulate the expression of associated genes. The thi-box riboswitch can exhibit a 1000-fold higher affinity for thiamine pyrophosphate over closely related noncognate compounds such as thiamine monophosphate. To understand the chemical basis of thi-box pyrophosphate specificity, we have determined crystal structures of an E. coli thi-box bound to thiamine pyrophosphate, thiamine monophosphate, and the structural analogs benfotiamine and pyrithiamine. When bound to monophosphorylated compounds, the RNA elements that recognize the thiamine and phosphate moieties of the ligand move closer together. This allows the riboswitch to recognize the monophosphate in a manner similar to how it recognizes the beta-phosphate of thiamine pyrophosphate. In the pyrithiamine complex, the pyrophosphate binding site is largely unstructured. These results show how the riboswitch can bind to various metabolites, and why the thi-box preferentially binds thiamine pyrophosphate.
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(as it appears on PubMed at http://www.pubmed.gov), where 16962976 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16962976}}
==About this Structure==
==About this Structure==
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[[Category: Riboswitch]]
[[Category: Riboswitch]]
[[Category: Rna]]
[[Category: Rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:31:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:04:05 2008''

Revision as of 14:04, 28 July 2008

Image:2hoo.png

Template:STRUCTURE 2hoo

Crystal structure of an E. coli thi-box riboswitch bound to benfotiamine

Template:ABSTRACT PUBMED 16962976

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition., Edwards TE, Ferre-D'Amare AR, Structure. 2006 Sep;14(9):1459-68. PMID:16962976

Page seeded by OCA on Mon Jul 28 17:04:05 2008

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