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| - | [[Image:1rz0.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1rz0| PDB=1rz0 | SCENE= }} | | {{STRUCTURE_1rz0| PDB=1rz0 | SCENE= }} |
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| - | '''Flavin reductase PheA2 in native state'''
| + | ===Flavin reductase PheA2 in native state=== |
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| - | ==Overview==
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| - | The catabolism of toxic phenols in the thermophilic organism Bacillus thermoglucosidasius A7 is initiated by a two-component enzyme system. The smaller flavin reductase PheA2 component catalyzes the NADH-dependent reduction of free FAD according to a ping-pong bisubstrate-biproduct mechanism. The reduced FAD is then used by the larger oxygenase component PheA1 to hydroxylate phenols to the corresponding catechols. We have determined the x-ray structure of PheA2 containing a bound FAD cofactor (2.2 A), which is the first structure of a member of this flavin reductase family. We have also determined the x-ray structure of reduced holo-PheA2 in complex with oxidized NAD (2.1 A). PheA2 is a single domain homodimeric protein with each FAD-containing subunit being organized around a six-stranded beta-sheet and a capping alpha-helix. The tightly bound FAD prosthetic group (K(d) = 10 nm) binds near the dimer interface, and the re face of the FAD isoalloxazine ring is fully exposed to solvent. The addition of NADH to crystalline PheA2 reduced the flavin cofactor, and the NAD product was bound in a wide solvent-accessible groove adopting an unusual folded conformation with ring stacking. This is the first observation of an enzyme that is very likely to react with a folded compact pyridine nucleotide. The PheA2 crystallographic models strongly suggest that reactive exogenous FAD substrate binds in the NADH cleft after release of NAD product. Nanoflow electrospray mass spectrometry data indeed showed that PheA2 is able to bind one FAD cofactor and one FAD substrate. In conclusion, the structural data provide evidence that PheA2 contains a dual binding cleft for NADH and FAD substrate, which alternate during catalysis.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_14703520}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14703520 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14703520}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Fad]] | | [[Category: Fad]] |
| | [[Category: Flavin]] | | [[Category: Flavin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:05:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:06:16 2008'' |
Revision as of 14:06, 28 July 2008
Template:STRUCTURE 1rz0
Flavin reductase PheA2 in native state
Template:ABSTRACT PUBMED 14703520
About this Structure
1RZ0 is a Single protein structure of sequence from Geobacillus thermoglucosidasius. Full crystallographic information is available from OCA.
Reference
Structural studies on flavin reductase PheA2 reveal binding of NAD in an unusual folded conformation and support novel mechanism of action., van den Heuvel RH, Westphal AH, Heck AJ, Walsh MA, Rovida S, van Berkel WJ, Mattevi A, J Biol Chem. 2004 Mar 26;279(13):12860-7. Epub 2003 Dec 31. PMID:14703520
Page seeded by OCA on Mon Jul 28 17:06:16 2008
