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2fja

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[[Image:2fja.gif|left|200px]]
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{{STRUCTURE_2fja| PDB=2fja | SCENE= }}
{{STRUCTURE_2fja| PDB=2fja | SCENE= }}
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'''adenosine 5'-phosphosulfate reductase in complex with substrate'''
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===adenosine 5'-phosphosulfate reductase in complex with substrate===
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==Overview==
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The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulfite-AMP) have been recently elucidated at 1.6 and 2.5 A resolution, respectively. Here we present new structural features of the enzyme trapped in four different catalytically relevant states that provide us with a detailed picture of its reaction cycle. In the oxidized state (FAD(ox)), the isoalloxazine moiety of the FAD cofactor exhibits a similarly bent conformation as observed in the structure of the reduced enzyme. In the APS-bound state (FAD(ox)-APS), the substrate APS is embedded into a 17 A long substrate channel in such a way that the isoalloxazine ring is pushed toward the channel bottom, thereby producing a compressed enzyme-substrate complex. A clamp formed by residues ArgA317 and LeuA278 to fix the adenine ring and the curved APS conformation appear to be key factors to hold APS in a strained conformation. This energy-rich state is relaxed during the attack of APS on the reduced FAD. A relaxed FAD-sulfite adduct is observed in the structure of the FAD-sulfite state. Finally, a FAD-sulfite-AMP1 state with AMP within van der Waals distance of the sulfite adduct could be characterized. This structure documents how adjacent negative charges are stabilized by the protein matrix which is crucial for forming APS from AMP and sulfite in the reverse reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 16503650 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16503650}}
==About this Structure==
==About this Structure==
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[[Category: Aps reductase]]
[[Category: Aps reductase]]
[[Category: Sulfur cycle]]
[[Category: Sulfur cycle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:57:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:26:37 2008''

Revision as of 14:26, 28 July 2008

Image:2fja.png

Template:STRUCTURE 2fja

adenosine 5'-phosphosulfate reductase in complex with substrate

Template:ABSTRACT PUBMED 16503650

About this Structure

2FJA is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5'-phosphosulfate reductase based on the structural characterization of different enzymatic states., Schiffer A, Fritz G, Kroneck PM, Ermler U, Biochemistry. 2006 Mar 7;45(9):2960-7. PMID:16503650

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