2jod

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2jod.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2jod.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2jod| PDB=2jod | SCENE= }}
{{STRUCTURE_2jod| PDB=2jod | SCENE= }}
-
'''Pac1-Rshort N-terminal EC domain Pacap(6-38) complex'''
+
===Pac1-Rshort N-terminal EC domain Pacap(6-38) complex===
-
==Overview==
+
<!--
-
The pituitary adenylate cyclase-activating polypeptide (PACAP) receptor is a class II G protein-coupled receptor that contributes to many different cellular functions including neurotransmission, neuronal survival, and synaptic plasticity. The solution structure of the potent antagonist PACAP (residues 6'-38') complexed to the N-terminal extracellular (EC) domain of the human splice variant hPAC1-R-short (hPAC1-R(S)) was determined by NMR. The PACAP peptide adopts a helical conformation when bound to hPAC1-R(S) with a bend at residue A18' and makes extensive hydrophobic and electrostatic interactions along the exposed beta-sheet and interconnecting loops of the N-terminal EC domain. Mutagenesis data on both the peptide and the receptor delineate the critical interactions between the C terminus of the peptide and the C terminus of the EC domain that define the high affinity and specificity of hormone binding to hPAC1-R(S). These results present a structural basis for hPAC1-R(S) selectivity for PACAP versus the vasoactive intestinal peptide and also differentiate PACAP residues involved in binding to the N-terminal extracellular domain versus other parts of the full-length hPAC1-R(S) receptor. The structural, mutational, and binding data are consistent with a model for peptide binding in which the C terminus of the peptide hormone interacts almost exclusively with the N-terminal EC domain, whereas the central region makes contacts to both the N-terminal and other extracellular parts of the receptor, ultimately positioning the N terminus of the peptide to contact the transmembrane region and result in receptor activation.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17470806}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17470806 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17470806}}
==About this Structure==
==About this Structure==
-
2JOD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOD OCA].
+
2JOD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JOD OCA].
==Reference==
==Reference==
Line 36: Line 40:
[[Category: Walter, K A.]]
[[Category: Walter, K A.]]
[[Category: Protein/peptide complex]]
[[Category: Protein/peptide complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:06:35 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:34:29 2008''

Revision as of 14:34, 28 July 2008

Template:STRUCTURE 2jod

Pac1-Rshort N-terminal EC domain Pacap(6-38) complex

Template:ABSTRACT PUBMED 17470806

About this Structure

2JOD is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

Solution structure and mutational analysis of pituitary adenylate cyclase-activating polypeptide binding to the extracellular domain of PAC1-RS., Sun C, Song D, Davis-Taber RA, Barrett LW, Scott VE, Richardson PL, Pereda-Lopez A, Uchic ME, Solomon LR, Lake MR, Walter KA, Hajduk PJ, Olejniczak ET, Proc Natl Acad Sci U S A. 2007 May 8;104(19):7875-80. Epub 2007 Apr 30. PMID:17470806

Page seeded by OCA on Mon Jul 28 17:34:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools