We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2r6s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2r6s", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Line 1: Line 1:
-
[[Image:2r6s.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2r6s.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2r6s| PDB=2r6s | SCENE= }}
{{STRUCTURE_2r6s| PDB=2r6s | SCENE= }}
-
'''Crystal structure of Gab protein'''
+
===Crystal structure of Gab protein===
-
==Overview==
+
<!--
-
In protein crystallography, monodisperse protein samples of high purity are usually required in order to obtain diffraction-quality crystals. Here, crystals were reproducibly grown from a protein sample before its homogeneity had been determined. The sample was obtained after the first attempt to purify a recombinant target protein from an Escherichia coli cell lysate. Subsequent analysis revealed that it was a mixture of about 50 different proteins with no predominant species. Diffraction data were collected to 2.1 A and the space group was identified as I422. A molecular-replacement search with models of the expected target did not give a solution, which suggested that a contaminating E. coli protein had been crystallized. A PDB search revealed 256 structures determined in space group I422, of which 14 are E. coli proteins and two have unit-cell parameters similar to those observed. Molecular replacement with these structures showed a clear solution for one of them, the Gab protein. The structure is presented and compared with the deposited structure, from which it shows small but significant differences. The refined model contains bicine and sulfate as bound ligands, which provide insights into possible substrate-binding sites.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18391407}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18391407 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18391407}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Jelly-roll motif]]
[[Category: Jelly-roll motif]]
[[Category: Unknown function]]
[[Category: Unknown function]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:52:24 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:38:13 2008''

Revision as of 14:38, 28 July 2008

Image:2r6s.png

Template:STRUCTURE 2r6s

Crystal structure of Gab protein

Template:ABSTRACT PUBMED 18391407

About this Structure

2R6S is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A mixture of fortunes: the curious determination of the structure of Escherichia coli BL21 Gab protein., Lohkamp B, Dobritzsch D, Acta Crystallogr D Biol Crystallogr. 2008 Apr;64(Pt 4):407-15. Epub 2008, Mar 19. PMID:18391407

Page seeded by OCA on Mon Jul 28 17:38:13 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r6s"
Personal tools