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1zi0

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{{STRUCTURE_1zi0| PDB=1zi0 | SCENE= }}
{{STRUCTURE_1zi0| PDB=1zi0 | SCENE= }}
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'''A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias'''
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===A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias===
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==Overview==
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DNA gyrase is unique among type II topoisomerases in that its DNA supercoiling activity is unidirectional. The C-terminal domain of the gyrase A subunit (GyrA-CTD) is required for this supercoiling bias. We report here the x-ray structure of the Escherichia coli GyrA-CTD (Protein Data Bank code 1ZI0). The E. coli GyrA-CTD adopts a circular-shaped beta-pinwheel fold first seen in the Borrelia burgdorferi GyrA-CTD. However, whereas the B. burgdorferi GyrA-CTD is flat, the E. coli GyrA-CTD is spiral. DNA relaxation assays reveal that the E. coli GyrA-CTD wraps DNA inducing substantial (+) superhelicity, while the B. burgdorferi GyrA-CTD introduces a more modest (+) superhelicity. The observation of a superhelical spiral in the present structure and that of the Bacillus stearothermophilus ParC-CTD structure suggests unexpected similarities in substrate selectivity between gyrase and Topo IV enzymes. We propose a model wherein the right-handed ((+) solenoidal) wrapping of DNA around the E. coli GyrA-CTD enforces unidirectional (-) DNA supercoiling.
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{{ABSTRACT_PUBMED_15897198}}
==About this Structure==
==About this Structure==
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[[Category: Spiralling beta pinwheel]]
[[Category: Spiralling beta pinwheel]]
[[Category: Topoisomerase]]
[[Category: Topoisomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:38:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:38:28 2008''

Revision as of 14:38, 28 July 2008

Image:1zi0.png

Template:STRUCTURE 1zi0

A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias

Template:ABSTRACT PUBMED 15897198

About this Structure

1ZI0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A superhelical spiral in the Escherichia coli DNA gyrase A C-terminal domain imparts unidirectional supercoiling bias., Ruthenburg AJ, Graybosch DM, Huetsch JC, Verdine GL, J Biol Chem. 2005 Jul 15;280(28):26177-84. Epub 2005 May 15. PMID:15897198

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