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1vbp

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{{STRUCTURE_1vbp| PDB=1vbp | SCENE= }}
{{STRUCTURE_1vbp| PDB=1vbp | SCENE= }}
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'''Crystal structure of artocarpin-mannopentose complex'''
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===Crystal structure of artocarpin-mannopentose complex===
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==Overview==
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Artocarpin, a tetrameric lectin of molecular mass 65 kDa, is one of the two lectins extracted from the seeds of jackfruit. The structures of the complexes of artocarpin with mannotriose and mannopentose reported here, together with the structures of artocarpin and its complex with Me-alpha-mannose reported earlier, show that the lectin possesses a deep-seated binding site formed by three loops. The binding site can be considered as composed of two subsites; the primary site and the secondary site. Interactions at the primary site composed of two of the loops involve mainly hydrogen bonds, while those at the secondary site comprising the third loop are primarily van der Waals in nature. Mannotriose in its complex with the lectin interacts through all the three mannopyranosyl residues; mannopentose interacts with the protein using at least three of the five mannose residues. The complexes provide a structural explanation for the carbohydrate specificities of artocarpin. A detailed comparison with the sugar complexes of heltuba, the only other mannose-specific jacalin-like lectin with known three-dimensional structure in sugar-bound form, establishes the role of the sugar-binding loop constituting the secondary site, in conferring different specificities at the oligosaccharide level. This loop is four residues longer in artocarpin than in heltuba, providing an instance where variation in loop length is used as a strategy for generating carbohydrate specificity.
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(as it appears on PubMed at http://www.pubmed.gov), where 15099743 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15099743}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity., Jeyaprakash AA, Srivastav A, Surolia A, Vijayan M, J Mol Biol. 2004 May 7;338(4):757-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15099743 15099743]
Structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity., Jeyaprakash AA, Srivastav A, Surolia A, Vijayan M, J Mol Biol. 2004 May 7;338(4):757-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15099743 15099743]
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Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity., Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Mar 22;317(2):237-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11902840 11902840]
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A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold., Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M, Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8673603 8673603]
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Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study., Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2003 Sep 5;332(1):217-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12946359 12946359]
[[Category: Jeyaprakash, A A.]]
[[Category: Jeyaprakash, A A.]]
[[Category: Srivastav, A.]]
[[Category: Srivastav, A.]]
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[[Category: Lectin]]
[[Category: Lectin]]
[[Category: Mannose-specific]]
[[Category: Mannose-specific]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:20:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:38:25 2008''

Revision as of 14:38, 28 July 2008

Image:1vbp.png

Template:STRUCTURE 1vbp

Crystal structure of artocarpin-mannopentose complex

Template:ABSTRACT PUBMED 15099743

About this Structure

Full crystallographic information is available from OCA.

Reference

Structural basis for the carbohydrate specificities of artocarpin: variation in the length of a loop as a strategy for generating ligand specificity., Jeyaprakash AA, Srivastav A, Surolia A, Vijayan M, J Mol Biol. 2004 May 7;338(4):757-70. PMID:15099743

Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity., Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Mar 22;317(2):237-47. PMID:11902840

A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold., Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M, Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:8673603

Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study., Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2003 Sep 5;332(1):217-28. PMID:12946359

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