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1nev

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{{STRUCTURE_1nev| PDB=1nev | SCENE= }}
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'''A-tract decamer'''
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===A-tract decamer===
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==Overview==
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DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing an adenine (A)(4)-tract and used to refine the structure. The overall bend in the molecule is a result of in-phase negative roll in the A-tract and positive roll at its 5' junction, as well as positive and negative tilt inside the A-tract and near its junctions. The bend magnitude and direction obtained from NMR structures is 9.0 degrees into the minor groove in a coordinate frame located at the third AT base pair. We evaluated long-range and wedge models for DNA curvature and concluded that our data for A-tract curvature are best explained by a "delocalized bend" model. The global bend magnitude and direction of the NMR structure are in excellent agreement with the junction model parameters used to rationalize gel electrophoretic data and with preliminary results of a cyclization kinetics assay from our laboratory.
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(as it appears on PubMed at http://www.pubmed.gov), where 12586860 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12586860}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NEV OCA].
==Reference==
==Reference==
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[[Category: Dna bending]]
[[Category: Dna bending]]
[[Category: Dna decamer]]
[[Category: Dna decamer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:26:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:41:06 2008''

Revision as of 14:41, 28 July 2008

Image:1nev.png

Template:STRUCTURE 1nev

A-tract decamer

Template:ABSTRACT PUBMED 12586860

About this Structure

Full experimental information is available from OCA.

Reference

Structural origins of adenine-tract bending., Barbic A, Zimmer DP, Crothers DM, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2369-73. Epub 2003 Feb 13. PMID:12586860

Page seeded by OCA on Mon Jul 28 17:41:06 2008

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