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| | {{STRUCTURE_1rmn| PDB=1rmn | SCENE= }} | | {{STRUCTURE_1rmn| PDB=1rmn | SCENE= }} |
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| - | '''A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS'''
| + | ===A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS=== |
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| - | ==Overview==
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| - | For the understanding of the catalytic function of the RNA hammerhead ribozyme, a three-dimensional model is essential but neither a crystal nor a solution structure has been available. Fluorescence resonance energy transfer (FRET) was used to study the structure of the ribozyme in solution in order to establish the relative spatial orientation of the three constituent Watson-Crick base-paired helical segments. Synthetic constructs were labeled with the fluorescence donor (5-carboxyfluorescein) and acceptor (5-carboxytetramethylrhodamine) located at the ends of the strands constituting the ribozyme molecule. The acceptor helix in helix pairs I and III and in II and III was varied in length from 5 to 11 and 5 to 9 base pairs, respectively, and the FRET efficiencies were determined and correlated with a reference set of labeled RNA duplexes. The FRET efficiencies were predicted on the basis of vector algebra analysis, as a function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a Y-shaped arrangement of the ribozyme with helices I and II in close proximity and helix III pointing away. These orientational constraints were used for molecular modeling of a three-dimensional structure of the complete ribozyme.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_7973630}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7973630 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7973630}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Tuschl, T.]] | | [[Category: Tuschl, T.]] |
| | [[Category: Westhof, E.]] | | [[Category: Westhof, E.]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:40:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:47:55 2008'' |
Revision as of 14:48, 28 July 2008
Template:STRUCTURE 1rmn
A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS
Template:ABSTRACT PUBMED 7973630
About this Structure
Full crystallographic information is available from OCA.
Reference
A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements., Tuschl T, Gohlke C, Jovin TM, Westhof E, Eckstein F, Science. 1994 Nov 4;266(5186):785-9. PMID:7973630
Page seeded by OCA on Mon Jul 28 17:47:55 2008
