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| | {{STRUCTURE_2j8r| PDB=2j8r | SCENE= }} | | {{STRUCTURE_2j8r| PDB=2j8r | SCENE= }} |
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| - | '''STRUCTURE OF P. AERUGINOSA ACETYLTRANSFERASE PA4866 SOLVED IN COMPLEX WITH L-METHIONINE SULFOXIMINE'''
| + | ===STRUCTURE OF P. AERUGINOSA ACETYLTRANSFERASE PA4866 SOLVED IN COMPLEX WITH L-METHIONINE SULFOXIMINE=== |
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| - | ==Overview==
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| - | The gene PA4866 from Pseudomonas aeruginosa is documented in the Pseudomonas genome database as encoding a 172 amino acid hypothetical acetyltransferase. We and others have described the 3D structure of this protein (termed pita) [Davies et al. (2005) Proteins: Struct., Funct., Bioinf. 61, 677-679; Nocek et al., unpublished results], and structures have also been reported for homologues from Agrobacterium tumefaciens (Rajashankar et al., unpublished results) and Bacillus subtilis [Badger et al. (2005) Proteins: Struct., Funct., Bioinf. 60, 787-796]. Pita homologues are found in a large number of bacterial genomes, and while the majority of these have been assigned putative phosphinothricin acetyltransferase activity, their true function is unknown. In this paper we report that pita has no activity toward phosphinothricin. Instead, we demonstrate that pita acts as an acetyltransferase using the glutamate analogues l-methionine sulfoximine and l-methionine sulfone as substrates, with Km(app) values of 1.3 +/- 0.21 and 1.3 +/- 0.13 mM and kcat(app) values of 505 +/- 43 and 610 +/- 23 s-1 for l-methionine sulfoximine and l-methionine sulfone, respectively. A high-resolution (1.55 A) crystal structure of pita in complex with one of these substrates (l-methionine sulfoximine) has been solved, revealing the mode of its interaction with the enzyme. Comparison with the apoenzyme structure has also revealed how certain active site residues undergo a conformational change upon substrate binding. To investigate the role of pita in P. aeruginosa, a mutant strain, Depp4, in which pita was inactivated through an in-frame deletion, was constructed by allelic exchange. Growth of strain Depp4 in the absence of glutamine was inhibited by l-methionine sulfoximine, suggesting a role for pita in protecting glutamine synthetase from inhibition. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17253769}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17253769 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17253769}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pseudomonas aeruginosa]] | | [[Category: Pseudomonas aeruginosa]] |
| | [[Category: Transferase]] | | [[Category: Transferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:31:12 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:49:16 2008'' |
Revision as of 14:49, 28 July 2008
Template:STRUCTURE 2j8r
STRUCTURE OF P. AERUGINOSA ACETYLTRANSFERASE PA4866 SOLVED IN COMPLEX WITH L-METHIONINE SULFOXIMINE
Template:ABSTRACT PUBMED 17253769
About this Structure
2J8R is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
l-Methionine sulfoximine, but not phosphinothricin, is a substrate for an acetyltransferase (gene PA4866) from Pseudomonas aeruginosa: structural and functional studies., Davies AM, Tata R, Beavil RL, Sutton BJ, Brown PR, Biochemistry. 2007 Feb 20;46(7):1829-39. Epub 2007 Jan 25. PMID:17253769
Page seeded by OCA on Mon Jul 28 17:49:16 2008
