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2qcy

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{{STRUCTURE_2qcy| PDB=2qcy | SCENE= }}
{{STRUCTURE_2qcy| PDB=2qcy | SCENE= }}
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'''Crystal Structure of a monomeric form of Severe Acute Respiratory Syndrome (SARS) 3C-like protease mutant'''
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===Crystal Structure of a monomeric form of Severe Acute Respiratory Syndrome (SARS) 3C-like protease mutant===
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==Overview==
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Unlike 3C protease, the SARS-CoV 3C-like protease (3CLpro) is only enzymatically active as a homodimer and its catalysis is under extensive regulation by the unique extra domain. Despite intense studies, two puzzles still remain: 1) how dimer-monomer switch is controlled; and 2) why dimerization is absolutely required for catalysis. Here we report the monomeric crystal structure of the SARS-CoV 3CLpro mutant R298A at a resolution of 1.75 A. Detailed analysis reveals that Arg298 serves as a key component for maintaining dimerization and consequently its mutation will trigger a cooperative switch from dimer to monomer. The monomeric enzyme is irreversibly inactivated because its catalytic machinery is frozen in the collapsed state, characteristic of the formation of a short 310-helix from an active-site loop. Remarkably, dimerization appears to be coupled to catalysis in 3CLpro through use of overlapped residues for two networks, one for dimerization and another for the catalysis.
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The line below this paragraph, {{ABSTRACT_PUBMED_18305031}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18305031 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18305031}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: N-finger]]
[[Category: N-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:45:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:00:10 2008''

Revision as of 15:00, 28 July 2008

Image:2qcy.jpg

Template:STRUCTURE 2qcy

Crystal Structure of a monomeric form of Severe Acute Respiratory Syndrome (SARS) 3C-like protease mutant

Template:ABSTRACT PUBMED 18305031

About this Structure

2QCY is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.

Reference

Mechanism for Controlling Dimer-monomer Switch and Coupling Dimerization to Catalysis of the SARS-CoV 3C-Like Protease., Shi J, Sivaraman J, Song J, J Virol. 2008 Feb 27;. PMID:18305031

Page seeded by OCA on Mon Jul 28 18:00:10 2008

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