1tkg

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{{STRUCTURE_1tkg| PDB=1tkg | SCENE= }}
{{STRUCTURE_1tkg| PDB=1tkg | SCENE= }}
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'''Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with an analog of seryladenylate'''
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===Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with an analog of seryladenylate===
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==Overview==
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The fidelity of aminoacylation of tRNA(Thr) by the threonyl-tRNA synthetase (ThrRS) requires the discrimination of the cognate substrate threonine from the noncognate serine. Misacylation by serine is corrected in a proofreading or editing step. An editing site has been located 39 A away from the aminoacylation site. We report the crystal structures of this editing domain in its apo form and in complex with the serine product, and with two nonhydrolyzable analogs of potential substrates: the terminal tRNA adenosine charged with serine, and seryl adenylate. The structures show how serine is recognized, and threonine rejected, and provide the structural basis for the editing mechanism, a water-mediated hydrolysis of the mischarged tRNA. When the adenylate analog binds in the editing site, a phosphate oxygen takes the place of one of the catalytic water molecules, thereby blocking the reaction. This rules out a correction mechanism that would occur before the binding of the amino acid on the tRNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_15525511}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15525511 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15525511}}
==About this Structure==
==About this Structure==
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[[Category: Torres-Larios, A.]]
[[Category: Torres-Larios, A.]]
[[Category: Ligase]]
[[Category: Ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:03:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:02:13 2008''

Revision as of 15:02, 28 July 2008

Image:1tkg.jpg

Template:STRUCTURE 1tkg

Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with an analog of seryladenylate

Template:ABSTRACT PUBMED 15525511

About this Structure

1TKG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Achieving error-free translation; the mechanism of proofreading of threonyl-tRNA synthetase at atomic resolution., Dock-Bregeon AC, Rees B, Torres-Larios A, Bey G, Caillet J, Moras D, Mol Cell. 2004 Nov 5;16(3):375-86. PMID:15525511

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