From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| | + | {{Seed}} |
| | [[Image:1oca.gif|left|200px]] | | [[Image:1oca.gif|left|200px]] |
| | | | |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1oca| PDB=1oca | SCENE= }} | | {{STRUCTURE_1oca| PDB=1oca | SCENE= }} |
| | | | |
| - | '''HUMAN CYCLOPHILIN A, UNLIGATED, NMR, 20 STRUCTURES'''
| + | ===HUMAN CYCLOPHILIN A, UNLIGATED, NMR, 20 STRUCTURES=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The nuclear magnetic resonance (NMR) solution structure of free, unligated cyclophilin A (CypA), which is an 18 kDa protein from human T-lymphocytes that was expressed in Escherichia coli for the present study, was determined using multidimensional heteronuclear NMR techniques. Sequence-specific resonance assignments for 99.5% of all backbone amide protons and non-labile hydrogen atoms provided the basis for collection of an input of 4101 nuclear Overhauser enhancement (NOE) upper distance constraints and 371 dihedral angle constraints for distance geometry calculations and energy minimization with the programs DIANA and OPAL. The average RMSD values of the 20 best energy-refined NMR conformers relative to the mean coordinates are 0.49 A for the backbone atoms and 0.88 A for all heavy atoms of residues 2 to 165. The molecular architecture includes an eight-stranded antiparallel beta-barrel that is closed by two amphipathic alpha-helices. Detailed comparisons with the crystal structure of free CypA revealed subtle but significant conformational differences that can in most cases be related to lattice contacts in the crystal structure. 15N spin relaxation times and NMR lineshape analyses for CypA in the free form and complexed with cyclosporin A (CsA) revealed transitions of polypeptide loops surrounding the ligand-binding site from locally flexible conformations in the free protein, some of which include well-defined conformational equilibria, to well-defined spatial arrangements in the CypA-CsA complex. Compared to the crystal structure of free CypA, where the ligand-binding area is extensively involved in lattice contacts, the NMR structure presents a highly relevant reference for studies of changes in structure and internal mobility of the binding pocket upon ligand binding, and possible consequences of this conformational variability for calcineurin recognition by the CypA-CsA complex. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9299338}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9299338 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_9299338}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | 1OCA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCA OCA]. | + | 1OCA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCA OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 29: |
Line 33: |
| | [[Category: Isomerase]] | | [[Category: Isomerase]] |
| | [[Category: Peptidyl-prolyl cis-trans isomerase]] | | [[Category: Peptidyl-prolyl cis-trans isomerase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:39:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:27:03 2008'' |
Revision as of 15:27, 28 July 2008
Template:STRUCTURE 1oca
HUMAN CYCLOPHILIN A, UNLIGATED, NMR, 20 STRUCTURES
Template:ABSTRACT PUBMED 9299338
About this Structure
1OCA is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
The NMR solution conformation of unligated human cyclophilin A., Ottiger M, Zerbe O, Guntert P, Wuthrich K, J Mol Biol. 1997 Sep 12;272(1):64-81. PMID:9299338
Page seeded by OCA on Mon Jul 28 18:27:03 2008
