2qyf

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{{STRUCTURE_2qyf| PDB=2qyf | SCENE= }}
{{STRUCTURE_2qyf| PDB=2qyf | SCENE= }}
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'''Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex'''
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===Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex===
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==Overview==
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The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31(comet) complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."
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The line below this paragraph, {{ABSTRACT_PUBMED_18022368}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18022368 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18022368}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
p31comet blocks Mad2 activation through structural mimicry., Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X, Cell. 2007 Nov 16;131(4):744-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18022368 18022368]
p31comet blocks Mad2 activation through structural mimicry., Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X, Cell. 2007 Nov 16;131(4):744-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18022368 18022368]
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The Mad2 spindle checkpoint protein has two distinct natively folded states., Luo X, Tang Z, Xia G, Wassmann K, Matsumoto T, Rizo J, Yu H, Nat Struct Mol Biol. 2004 Apr;11(4):338-45. Epub 2004 Mar 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15024386 15024386]
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The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20., Luo X, Tang Z, Rizo J, Yu H, Mol Cell. 2002 Jan;9(1):59-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11804586 11804586]
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Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20., Luo X, Fang G, Coldiron M, Lin Y, Yu H, Kirschner MW, Wagner G, Nat Struct Biol. 2000 Mar;7(3):224-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700282 10700282]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
[[Category: Spindle assembly checkpoint]]
[[Category: Spindle assembly checkpoint]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:54:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:27:18 2008''

Revision as of 15:27, 28 July 2008

Image:2qyf.jpg

Template:STRUCTURE 2qyf

Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex

Template:ABSTRACT PUBMED 18022368

About this Structure

2QYF is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

p31comet blocks Mad2 activation through structural mimicry., Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X, Cell. 2007 Nov 16;131(4):744-55. PMID:18022368

The Mad2 spindle checkpoint protein has two distinct natively folded states., Luo X, Tang Z, Xia G, Wassmann K, Matsumoto T, Rizo J, Yu H, Nat Struct Mol Biol. 2004 Apr;11(4):338-45. Epub 2004 Mar 14. PMID:15024386

The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20., Luo X, Tang Z, Rizo J, Yu H, Mol Cell. 2002 Jan;9(1):59-71. PMID:11804586

Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20., Luo X, Fang G, Coldiron M, Lin Y, Yu H, Kirschner MW, Wagner G, Nat Struct Biol. 2000 Mar;7(3):224-9. PMID:10700282

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