1njq

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NMR structure of the single QALGGH zinc finger domain from Arabidopsis thaliana SUPERMAN protein

Overview

Zinc finger domains of the classical type represent the most abundant DNA, binding domains in eukaryotic transcription factors. Plant proteins, contain from one to four zinc finger domains, which are characterized by, high conservation of the sequence QALGGH, shown to be critical for, DNA-binding activity. The Arabidopsis thaliana SUPERMAN protein, which, contains a single QALGGH zinc finger, is necessary for proper spatial, development of reproductive floral tissues and has been shown to, specifically bind to DNA. Here, we report the synthesis and UV and NMR, spectroscopic structural characterization of a 37 amino acid SUPERMAN, region complexed to a Zn(2+) ion (Zn-SUP37) and present the first, high-resolution structure of a classical zinc finger domain from a plant, protein. The NMR structure of the SUPERMAN zinc finger domain consists of, a very well-defined betabetaalpha motif, typical of all other, Cys(2)-His(2) zinc fingers structurally characterized. As a consequence, the highly conserved QALGGH sequence is located at the N terminus of the, alpha helix. This region of the domain of animal zinc finger proteins, consists of hypervariable residues that are responsible for recognizing, the DNA bases. Therefore, we propose a peculiar DNA recognition code for, the QALGGH zinc finger domain that includes all or some of the amino acid, residues at positions -1, 2, and 3 (numbered relative to the N terminus of, the helix) and possibly others at the C-terminal end of the recognition, helix. This study further confirms that the zinc finger domain, though, very simple, is an extremely versatile DNA binding motif.

About this Structure

1NJQ is a Single protein structure of sequence from [1] with ZN, ACE and NH2 as ligands. Full crystallographic information is available from OCA.

Reference

NMR structure of the single QALGGH zinc finger domain from the Arabidopsis thaliana SUPERMAN protein., Isernia C, Bucci E, Leone M, Zaccaro L, Di Lello P, Digilio G, Esposito S, Saviano M, Di Blasio B, Pedone C, Pedone PV, Fattorusso R, Chembiochem. 2003 Mar 3;4(2-3):171-80. PMID:12616630

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