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2hk6

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{{STRUCTURE_2hk6| PDB=2hk6 | SCENE= }}
{{STRUCTURE_2hk6| PDB=2hk6 | SCENE= }}
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'''Crystal Structure of B. subtilis ferrochelatase with Iron bound at the active site'''
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===Crystal Structure of B. subtilis ferrochelatase with Iron bound at the active site===
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==Overview==
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Ferrochelatase catalyzes the terminal step in the heme biosynthetic pathway, i.e., the incorporation of Fe(II) into protoporphyrin IX. Various biochemical and biophysical methods have been used to probe the enzyme for metal binding residues and the location of the active site. However, the location of the metal binding site and the path of the metal into the porphyrin are still disputed. Using site-directed mutagenesis on Bacillus subtilis ferrochelatase we demonstrate that exchange of the conserved residues His183 and Glu264 affects the metal affinity of the enzyme. We also present the first X-ray crystal structure of ferrochelatase with iron. Only a single iron was found in the active site, coordinated in a square pyramidal fashion by two amino acid residues, His183 and Glu264, and three water molecules. This iron was not present in the structure of a His183Ala modified ferrochelatase. The results strongly suggest that the insertion of a metal ion into protoporphyrin IX by ferrochelatase occurs from a metal binding site represented by His183 and Glu264.
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{{ABSTRACT_PUBMED_17198378}}
==About this Structure==
==About this Structure==
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[[Category: Metal-binding]]
[[Category: Metal-binding]]
[[Category: Porphyrin biosynthesis]]
[[Category: Porphyrin biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:23:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:01:46 2008''

Revision as of 16:01, 28 July 2008

Image:2hk6.png

Template:STRUCTURE 2hk6

Crystal Structure of B. subtilis ferrochelatase with Iron bound at the active site

Template:ABSTRACT PUBMED 17198378

About this Structure

2HK6 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Amino acid residues His183 and Glu264 in Bacillus subtilis ferrochelatase direct and facilitate the insertion of metal ion into protoporphyrin IX., Hansson MD, Karlberg T, Rahardja MA, Al-Karadaghi S, Hansson M, Biochemistry. 2007 Jan 9;46(1):87-94. PMID:17198378

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