2oxv

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[[Image:2oxv.gif|left|200px]]
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{{STRUCTURE_2oxv| PDB=2oxv | SCENE= }}
{{STRUCTURE_2oxv| PDB=2oxv | SCENE= }}
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'''Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.'''
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===Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.===
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==Overview==
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Promiscuous mutant EcoRI endonucleases bind to the canonical site GAATTC more tightly than does the wild-type endonuclease, yet cleave variant (EcoRI(*)) sites more rapidly than does wild-type. The crystal structure of the A138T promiscuous mutant homodimer in complex with a GAATTC site is nearly identical to that of the wild-type complex, except that the Thr138 side chains make packing interactions with bases in the 5'-flanking regions outside the recognition hexanucleotide while excluding two bound water molecules seen in the wild-type complex. Molecular dynamics simulations confirm exclusion of these waters. The structure and simulations suggest possible reasons why binding of the A138T protein to the GAATTC site has DeltaS degrees more favorable and DeltaH degrees less favorable than for wild-type endonuclease binding. The interactions of Thr138 with flanking bases may permit A138T, unlike wild-type enzyme, to form complexes with EcoRI(*) sites that structurally resemble the specific wild-type complex with GAATTC.
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The line below this paragraph, {{ABSTRACT_PUBMED_17997963}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17997963 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17997963}}
==About this Structure==
==About this Structure==
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[[Category: Relaxed specificity mutant]]
[[Category: Relaxed specificity mutant]]
[[Category: Type ii restriction endonuclease]]
[[Category: Type ii restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:53:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:02:26 2008''

Revision as of 16:02, 28 July 2008

Image:2oxv.png

Template:STRUCTURE 2oxv

Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.

Template:ABSTRACT PUBMED 17997963

About this Structure

2OXV is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease., Sapienza PJ, Rosenberg JM, Jen-Jacobson L, Structure. 2007 Nov;15(11):1368-82. PMID:17997963

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