1nnd
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(New page: 200px<br /><applet load="1nnd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nnd, resolution 2.30Å" /> '''Arginine 116 is Esse...)
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Revision as of 20:17, 20 November 2007
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Arginine 116 is Essential for Nucleic Acid Recognition by the Fingers Domain of Moloney Murine Leukemia Virus Reverse Transcriptase
Overview
Reverse transcriptase is an essential retroviral enzyme that replicates, the single-stranded RNA genome of the retrovirus producing a, double-stranded DNA copy, which is subsequently integrated into the host's, genome. We have previously reported that processive DNA synthesis of, Moloney murine leukemia virus reverse transcriptase (MMLV RT) is severely, compromised by substitution of an Ala for the fingers domain residue Arg, 116. In order to further investigate the role of Arg 116 in interactions, of MMLV RT with nucleic acids, we have determined the crystal structure of, the R116A N-terminal fragment and characterized the binding of two, self-complementary DNA duplexes [d(CATGCATG)2 and d(CGCGCGCG)2] to both, the wild-type and R116A fragments by isothermal titration calorimetry. The, resultant thermodynamic profiles extrapolated to 25 degrees C reveal that, binding of the wild-type N-terminal fragment to both DNA duplexes is, enthalpy-driven and characterized by an unfavorable entropy. Although the, temperature dependence of the respective protein-DNA binding enthalpies is, markedly different reflecting distinct heat capacity changes, the binding, free energies are nearly identical and relatively invariant to temperature, (DeltaG approximately -6.0 kcal x mol(-1)). In contrast to the wild-type, fragment, the R116A fragment exhibits no measurable affinity for either, DNA duplex, yet its crystal structure reveals no significant changes when, compared to the wild-type structures. We suggest that hydrogen-bonding, interactions involving the fingers domain residue Arg 116 are critical for, DNA binding as well as processive DNA synthesis by MMLV RT.
About this Structure
1NND is a Single protein structure of sequence from Moloney murine leukemia virus. Active as RNA-directed DNA polymerase, with EC number 2.7.7.49 Full crystallographic information is available from OCA.
Reference
Structural and energetic characterization of nucleic acid-binding to the fingers domain of Moloney murine leukemia virus reverse transcriptase., Crowther RL, Remeta DP, Minetti CA, Das D, Montano SP, Georgiadis MM, Proteins. 2004 Oct 1;57(1):15-26. PMID:15326591
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