1sjg

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{{STRUCTURE_1sjg| PDB=1sjg | SCENE= }}
{{STRUCTURE_1sjg| PDB=1sjg | SCENE= }}
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'''Solution Structure of T4moC, the Rieske Ferredoxin Component of the Toluene 4-Monooxygenase Complex'''
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===Solution Structure of T4moC, the Rieske Ferredoxin Component of the Toluene 4-Monooxygenase Complex===
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==Overview==
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Toluene 4-monooxygenase, a four-protein complex from Pseudomonas mendocina KR1, catalyzes the NADH- and O(2)-dependent hydroxylation of toluene to form p-cresol. The solution structure of the 112-amino-acid Rieske ferredoxin component, T4moC, was determined from 2D and 3D (1)H, (13)C, and (15)N NMR data. The structural model was refined through simulated annealing by molecular dynamics in torsion angle space with input from 1650 experimental restraints, including 1264 inter-proton distance restraints obtained from NOEs, 247 non-redundant intra-residue NOEs, 26 hydrogen bond restraints, and 113 dihedral angle ( phi, psi) restraints. The 20 calculated conformers that best satisfied the input restraints were submitted to refinement in explicit solvent to improve the stereochemical quality. With exclusion of ill-defined N- and C-terminal segments (Ser2; His111-Ser112) and residues near to the [2Fe-2S] cluster, the atomic root mean square deviation for the 20 conformers with respect to the mean coordinates was 1.09 A for the backbone and 1.60 A for all non-hydrogen atoms. The T4moC structure consists of 10 beta-strands arranged in the three anti-parallel beta-sheet topology observed in all Rieske [2Fe-2S] domain proteins. The S(gamma) of Cys45 and Cys64 and the N(delta1) of His47 and His67 provide the ligands to the [2Fe-2S] cluster of T4moC. (1)H-(15)N HSQC measurements show that both His47-N(epsilon2) and His67-N(epsilon2) are protonated at the pH of the NMR experiments. Comparisons are made between the present NMR structure, previous paramagnetic NMR studies of T4moC, and the X-ray structures of other members of the Rieske protein family.
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{{ABSTRACT_PUBMED_15452777}}
==About this Structure==
==About this Structure==
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1SJG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJG OCA].
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1SJG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJG OCA].
==Reference==
==Reference==
Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex., Skjeldal L, Peterson FC, Doreleijers JF, Moe LA, Pikus JD, Westler WM, Markley JL, Volkman BF, Fox BG, J Biol Inorg Chem. 2004 Dec;9(8):945-53. Epub 2004 Sep 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15452777 15452777]
Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex., Skjeldal L, Peterson FC, Doreleijers JF, Moe LA, Pikus JD, Westler WM, Markley JL, Volkman BF, Fox BG, J Biol Inorg Chem. 2004 Dec;9(8):945-53. Epub 2004 Sep 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15452777 15452777]
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Recombinant toluene-4-monooxygenase: catalytic and Mossbauer studies of the purified diiron and rieske components of a four-protein complex., Pikus JD, Studts JM, Achim C, Kauffmann KE, Munck E, Steffan RJ, McClay K, Fox BG, Biochemistry. 1996 Jul 16;35(28):9106-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703915 8703915]
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Detection and classification of hyperfine-shifted 1H, 2H, and 15N resonances of the Rieske ferredoxin component of toluene 4-monooxygenase., Xia B, Pikus JD, Xia W, McClay K, Steffan RJ, Chae YK, Westler WM, Markley JL, Fox BG, Biochemistry. 1999 Jan 12;38(2):727-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9888813 9888813]
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Application of fed-batch fermentation to the preparation of isotopically labeled or selenomethionyl-labeled proteins., Studts JM, Fox BG, Protein Expr Purif. 1999 Jun;16(1):109-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10336868 10336868]
[[Category: Pseudomonas mendocina]]
[[Category: Pseudomonas mendocina]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Ferredoxin]]
[[Category: Ferredoxin]]
[[Category: Pfam pf00355]]
[[Category: Pfam pf00355]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:16:57 2008''

Revision as of 16:16, 28 July 2008

Image:1sjg.png

Template:STRUCTURE 1sjg

Solution Structure of T4moC, the Rieske Ferredoxin Component of the Toluene 4-Monooxygenase Complex

Template:ABSTRACT PUBMED 15452777

About this Structure

1SJG is a Single protein structure of sequence from Pseudomonas mendocina. Full experimental information is available from OCA.

Reference

Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex., Skjeldal L, Peterson FC, Doreleijers JF, Moe LA, Pikus JD, Westler WM, Markley JL, Volkman BF, Fox BG, J Biol Inorg Chem. 2004 Dec;9(8):945-53. Epub 2004 Sep 25. PMID:15452777

Recombinant toluene-4-monooxygenase: catalytic and Mossbauer studies of the purified diiron and rieske components of a four-protein complex., Pikus JD, Studts JM, Achim C, Kauffmann KE, Munck E, Steffan RJ, McClay K, Fox BG, Biochemistry. 1996 Jul 16;35(28):9106-19. PMID:8703915

Detection and classification of hyperfine-shifted 1H, 2H, and 15N resonances of the Rieske ferredoxin component of toluene 4-monooxygenase., Xia B, Pikus JD, Xia W, McClay K, Steffan RJ, Chae YK, Westler WM, Markley JL, Fox BG, Biochemistry. 1999 Jan 12;38(2):727-39. PMID:9888813

Application of fed-batch fermentation to the preparation of isotopically labeled or selenomethionyl-labeled proteins., Studts JM, Fox BG, Protein Expr Purif. 1999 Jun;16(1):109-19. PMID:10336868

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