1uxd

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[[Image:1uxd.jpg|left|200px]]
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{{Seed}}
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[[Image:1uxd.png|left|200px]]
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{{STRUCTURE_1uxd| PDB=1uxd | SCENE= }}
{{STRUCTURE_1uxd| PDB=1uxd | SCENE= }}
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'''FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, 34 STRUCTURES'''
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===FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, 34 STRUCTURES===
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==Overview==
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FruR is an Escherichia coli transcriptional regulator that belongs to the LacI DNA-binding protein family. By using 1H and 15N NMR spectroscopy, we have determined the three-dimensional solution structure of the FruR N-terminal DNA-binding domain consisting of 57 amino acid residues. A total of 809 NMR-derived distances and 54 dihedral angle constraints have been used for molecular modelling with the X-PLOR program. The resulting set of calculated structures presents an average root-mean-square deviation of 0.37 A at the main-chain level for the first 47 residues. This highly defined N-terminal part of the structure reveals a similar topology for the three alpha-helices when compared to the 3D structures of LacI and PurR counterparts. The most striking difference lies in the connection between helix II and helix III, in which three additional residues are present in FruR. This connecting segment is well structured and contains a type III turn. Apart from hydrophobic interactions of non-polar residues with the core of the domain, this connecting segment is stabilised by several hydrogen bonds and by the aromatic ring stacking between Tyr19 of helix II and Tyr28 of the turn. The region containing the putative "hinge helix" (helix IV), that has been described in PurR-DNA complex to make specific base contacts in the minor groove of DNA, is unfolded. Examination of hydrogen bonds highlights the importance of homologous residues that seem to be conserved for their ability to fulfill helix N and C-capping roles in the LacI repressor family.
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{{ABSTRACT_PUBMED_9237914}}
==About this Structure==
==About this Structure==
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1UXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXD OCA].
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1UXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXD OCA].
==Reference==
==Reference==
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[[Category: Laci family]]
[[Category: Laci family]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:48:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:17:17 2008''

Revision as of 16:17, 28 July 2008

Image:1uxd.png

Template:STRUCTURE 1uxd

FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, 34 STRUCTURES

Template:ABSTRACT PUBMED 9237914

About this Structure

1UXD is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Three-dimensional structure of the DNA-binding domain of the fructose repressor from Escherichia coli by 1H and 15N NMR., Penin F, Geourjon C, Montserret R, Bockmann A, Lesage A, Yang YS, Bonod-Bidaud C, Cortay JC, Negre D, Cozzone AJ, Deleage G, J Mol Biol. 1997 Jul 18;270(3):496-510. PMID:9237914

Page seeded by OCA on Mon Jul 28 19:17:17 2008

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