2dwk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2dwk.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2dwk.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2dwk| PDB=2dwk | SCENE= }}
{{STRUCTURE_2dwk| PDB=2dwk | SCENE= }}
-
'''Crystal structure of the RUN domain of mouse Rap2 interacting protein x'''
+
===Crystal structure of the RUN domain of mouse Rap2 interacting protein x===
-
==Overview==
+
<!--
-
Rap2-interacting protein x (RPIPx) is a homolog of RPIP8, a specific effector of Rap2 GTPase. The N-terminal region of RPIP8, which contains the RUN domain, interacts with Rap2. Using cell-free synthesis and NMR, we determined that the region encompassing residues 83-255 of mouse RPIPx, which is 40-residues larger than the predicted RUN domain (residues 113-245), is the minimum fragment that forms a correctly folded protein. This fragment, the RPIPx RUN domain, interacted specifically with Rap2B in vitro in a nucleotide-dependent manner. The crystal structure of the RPIPx RUN domain was determined at 2.0 A of resolution by the multiwavelength anomalous dispersion (MAD) method. The RPIPx RUN domain comprises eight anti-parallel alpha-helices, which form an extensive hydrophobic core, followed by an extended segment. The residues in the core region are highly conserved, suggesting the conservation of the RUN domain-fold among the RUN domain-containing proteins. The residues forming a positively charged surface are conserved between RPIP8 and its homologs, suggesting that this surface is important for Rap2 binding. In the crystal the putative Rap2 binding site of the RPIPx RUN domain interacts with the extended segment in a segment-swapping manner.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16928684}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16928684 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16928684}}
==About this Structure==
==About this Structure==
Line 37: Line 41:
[[Category: Run domain]]
[[Category: Run domain]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:31:31 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:26:57 2008''

Revision as of 16:26, 28 July 2008

Image:2dwk.png

Template:STRUCTURE 2dwk

Crystal structure of the RUN domain of mouse Rap2 interacting protein x

Template:ABSTRACT PUBMED 16928684

About this Structure

2DWK is a Single protein structure of sequence from Mus musculus. This structure supersedes the now removed PDB entry 1wus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the RUN domain of the RAP2-interacting protein x., Kukimoto-Niino M, Takagi T, Akasaka R, Murayama K, Uchikubo-Kamo T, Terada T, Inoue M, Watanabe S, Tanaka A, Hayashizaki Y, Kigawa T, Shirouzu M, Yokoyama S, J Biol Chem. 2006 Oct 20;281(42):31843-53. Epub 2006 Aug 23. PMID:16928684

Page seeded by OCA on Mon Jul 28 19:26:57 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2dwk"
Personal tools