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2ifs

From Proteopedia

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{{STRUCTURE_2ifs| PDB=2ifs | SCENE= }}
{{STRUCTURE_2ifs| PDB=2ifs | SCENE= }}
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'''Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide'''
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===Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide===
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==Overview==
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The WASP-interacting protein (WIP) targets WASP/WAVE proteins through a constitutive interaction with an amino-terminal enabled/VASP homology (EVH1) domain. Parallel investigations had previously identified two distinct N-WASP binding motifs corresponding to WIP residues 451-461 and 461-485, and we determined the structure of a complex between WIP-(461-485) and the N-WASP EVH1 domain (Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C., and Lim, W. A. (2002) Cell 111, 565-576). The present results show that, when combined, the WIP-(451-485) sequence wraps further around the EVH1 domain, extending the interface observed previously. Specific contacts with three WIP epitopes corresponded to regions of high sequence conservation in the verprolin family. A central polyproline motif occupied the canonical binding site but in a reversed orientation relative to other EVH1 complexes. This interaction was augmented in the amino- and carboxyl-terminal directions by additional hydrophobic contacts involving WIP residues 454-459 and 475-478, respectively. Disruption of any of the three WIP epitopes reduced N-WASP binding in cells, demonstrating a functional requirement for the entire binding domain, which is significantly longer than the polyproline motifs recognized by other EVH1 domains.
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{{ABSTRACT_PUBMED_17229736}}
==About this Structure==
==About this Structure==
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2IFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_rattus_norvegicus Homo sapiens, rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA].
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2IFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_rattus_norvegicus Homo sapiens, rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA].
==Reference==
==Reference==
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[[Category: Verprolin]]
[[Category: Verprolin]]
[[Category: Wiskott-aldrich syndrome]]
[[Category: Wiskott-aldrich syndrome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:27:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:27:22 2008''

Revision as of 16:27, 28 July 2008

Image:2ifs.png

Template:STRUCTURE 2ifs

Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide

Template:ABSTRACT PUBMED 17229736

About this Structure

2IFS is a Single protein structure of sequence from Homo sapiens, rattus norvegicus. Full experimental information is available from OCA.

Reference

Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP., Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF, J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:17229736

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