2ifs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ifs.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2ifs.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ifs| PDB=2ifs | SCENE= }}
{{STRUCTURE_2ifs| PDB=2ifs | SCENE= }}
-
'''Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide'''
+
===Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide===
-
==Overview==
+
<!--
-
The WASP-interacting protein (WIP) targets WASP/WAVE proteins through a constitutive interaction with an amino-terminal enabled/VASP homology (EVH1) domain. Parallel investigations had previously identified two distinct N-WASP binding motifs corresponding to WIP residues 451-461 and 461-485, and we determined the structure of a complex between WIP-(461-485) and the N-WASP EVH1 domain (Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C., and Lim, W. A. (2002) Cell 111, 565-576). The present results show that, when combined, the WIP-(451-485) sequence wraps further around the EVH1 domain, extending the interface observed previously. Specific contacts with three WIP epitopes corresponded to regions of high sequence conservation in the verprolin family. A central polyproline motif occupied the canonical binding site but in a reversed orientation relative to other EVH1 complexes. This interaction was augmented in the amino- and carboxyl-terminal directions by additional hydrophobic contacts involving WIP residues 454-459 and 475-478, respectively. Disruption of any of the three WIP epitopes reduced N-WASP binding in cells, demonstrating a functional requirement for the entire binding domain, which is significantly longer than the polyproline motifs recognized by other EVH1 domains.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17229736}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17229736 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17229736}}
==About this Structure==
==About this Structure==
-
2IFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_rattus_norvegicus Homo sapiens, rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA].
+
2IFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_rattus_norvegicus Homo sapiens, rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA].
==Reference==
==Reference==
Line 30: Line 34:
[[Category: Verprolin]]
[[Category: Verprolin]]
[[Category: Wiskott-aldrich syndrome]]
[[Category: Wiskott-aldrich syndrome]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:27:10 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:27:22 2008''

Revision as of 16:27, 28 July 2008

Image:2ifs.png

Template:STRUCTURE 2ifs

Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide

Template:ABSTRACT PUBMED 17229736

About this Structure

2IFS is a Single protein structure of sequence from Homo sapiens, rattus norvegicus. Full experimental information is available from OCA.

Reference

Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP., Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF, J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:17229736

Page seeded by OCA on Mon Jul 28 19:27:22 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ifs"
Personal tools