This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2epo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2epo.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2epo.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2epo| PDB=2epo | SCENE= }}
{{STRUCTURE_2epo| PDB=2epo | SCENE= }}
-
'''N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii'''
+
===N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii===
-
==Overview==
+
<!--
-
The crystal structure of GcnA, an N-acetyl-beta-D-glucosaminidase from Streptococcus gordonii, was solved by multiple wavelength anomalous dispersion phasing using crystals of selenomethionine-substituted protein. GcnA is a homodimer with subunits each comprised of three domains. The structure of the C-terminal alpha-helical domain has not been observed previously and forms a large dimerisation interface. The fold of the N-terminal domain is observed in all structurally related glycosidases although its function is unknown. The central domain has a canonical (beta/alpha)(8) TIM-barrel fold which harbours the active site. The primary sequence and structure of this central domain identifies the enzyme as a family 20 glycosidase. Key residues implicated in catalysis have different conformations in two different crystal forms, which probably represent active and inactive conformations of the enzyme. The catalytic mechanism for this class of glycoside hydrolase, where the substrate rather than the enzyme provides the cleavage-inducing nucleophile, has been confirmed by the structure of GcnA complexed with a putative reaction intermediate analogue, N-acetyl-beta-D-glucosamine-thiazoline. The catalytic mechanism is discussed in light of these and other family 20 structures.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18237743}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18237743 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18237743}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Glucosaminidase]]
[[Category: Glucosaminidase]]
[[Category: Glycoside hydrolase]]
[[Category: Glycoside hydrolase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:57:24 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:28:16 2008''

Revision as of 16:28, 28 July 2008

Image:2epo.png

Template:STRUCTURE 2epo

N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii

Template:ABSTRACT PUBMED 18237743

About this Structure

2EPO is a Single protein structure of sequence from Streptococcus gordonii. Full crystallographic information is available from OCA.

Reference

Structure of N-acetyl-beta-D-glucosaminidase (GcnA) from the endocarditis pathogen Streptococcus gordonii and its complex with the mechanism-based inhibitor NAG-thiazoline., Langley DB, Harty DW, Jacques NA, Hunter N, Guss JM, Collyer CA, J Mol Biol. 2008 Mar 14;377(1):104-16. Epub 2007 Sep 16. PMID:18237743

Page seeded by OCA on Mon Jul 28 19:28:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2epo"
Personal tools