2mhu

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[[Image:2mhu.jpg|left|200px]]
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{{Seed}}
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[[Image:2mhu.png|left|200px]]
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{{STRUCTURE_2mhu| PDB=2mhu | SCENE= }}
{{STRUCTURE_2mhu| PDB=2mhu | SCENE= }}
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'''THE THREE-DIMENSIONAL STRUCTURE OF HUMAN [113CD7] METALLOTHIONEIN-2 IN SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY'''
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===THE THREE-DIMENSIONAL STRUCTURE OF HUMAN [113CD7] METALLOTHIONEIN-2 IN SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY===
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==Overview==
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The three-dimensional structure of human [113Cd7]metallothionein-2 was determined by nuclear magnetic resonance spectroscopy in solution. Sequence-specific 1H resonance assignments were obtained using the sequential assignment method. The input for the structure calculations consisted of the metal-cysteine co-ordinative bonds identified with heteronuclear correlation spectroscopy, 1H-1H distance constraints from nuclear Overhauser enhancement spectroscopy, and spin-spin coupling constants 3JHN alpha and 3J alpha beta. The molecule consists of two domains, the beta-domain including amino acid residues 1 to 30 and three metal ions, and the alpha-domain including residues 31 to 61 and four metal ions. The nuclear magnetic resonance data present no evidence for a preferred relative orientation of the two domains. The polypeptide-to-metal co-ordinative bonds in human metallothionein-2 are identical to those in the previously determined solution structures of rat metallothionein-2 and rabbit metallothionein-2a, and the polypeptide conformations in the three proteins are also closely similar.
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The line below this paragraph, {{ABSTRACT_PUBMED_2388267}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 2388267 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2388267}}
==About this Structure==
==About this Structure==
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2MHU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MHU OCA].
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2MHU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MHU OCA].
==Reference==
==Reference==
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[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
[[Category: Metallothionein]]
[[Category: Metallothionein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:33:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:33:47 2008''

Revision as of 16:33, 28 July 2008

Image:2mhu.png

Template:STRUCTURE 2mhu

THE THREE-DIMENSIONAL STRUCTURE OF HUMAN [113CD7] METALLOTHIONEIN-2 IN SOLUTION DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Template:ABSTRACT PUBMED 2388267

About this Structure

2MHU is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Three-dimensional structure of human [113Cd7]metallothionein-2 in solution determined by nuclear magnetic resonance spectroscopy., Messerle BA, Schaffer A, Vasak M, Kagi JH, Wuthrich K, J Mol Biol. 1990 Aug 5;214(3):765-79. PMID:2388267

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