2o9u

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{{STRUCTURE_2o9u| PDB=2o9u | SCENE= }}
{{STRUCTURE_2o9u| PDB=2o9u | SCENE= }}
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'''Monellin (MNEI) at 1.15 resolution'''
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===Monellin (MNEI) at 1.15 resolution===
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==Overview==
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The X-ray crystal structure of a single-chain monellin protein (MNEI) has been determined at 1.15 A resolution. The model was refined to convergence employing anisotropic displacement parameters and riding H atoms to produce a final model with R(work) and R(free) values of 0.132 and 0.162, respectively. The crystal contains a single MNEI protein in the asymmetric unit and unusually lacks the dimer interface observed in all previous crystal structures of monellin and its single-chain derivatives. The high resolution allowed a more detailed view of MNEI than previously possible, with 38 of the 96 residues modelled with alternative side-chain conformations, including four core residues Thr12, Cys41, Leu62 and Ile75. Four stably bound negative ions were also located, providing new insight into potential electrostatic interactions of MNEI with the largely negatively charged surface of the sweet taste receptor T1R2-T1R3.
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(as it appears on PubMed at http://www.pubmed.gov), where 17329805 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17329805}}
==About this Structure==
==About this Structure==
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[[Category: Alternative conformation]]
[[Category: Alternative conformation]]
[[Category: Plant protein]]
[[Category: Plant protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:16:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:39:42 2008''

Revision as of 16:39, 28 July 2008

Image:2o9u.png

Template:STRUCTURE 2o9u

Monellin (MNEI) at 1.15 resolution

Template:ABSTRACT PUBMED 17329805

About this Structure

2O9U is a Single protein structure of sequence from Dioscoreophyllum cumminsii. Full crystallographic information is available from OCA.

Reference

Monellin (MNEI) at 1.15 A resolution., Hobbs JR, Munger SD, Conn GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):162-7. Epub 2007 Feb 13. PMID:17329805

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