This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ueo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ueo.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1ueo.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ueo| PDB=1ueo | SCENE= }}
{{STRUCTURE_1ueo| PDB=1ueo | SCENE= }}
-
'''Solution structure of the [T8A]-Penaeidin-3'''
+
===Solution structure of the [T8A]-Penaeidin-3===
-
==Overview==
+
<!--
-
Penaeidins are a family of antimicrobial peptides of 47-63 residues isolated from several species of shrimp. These peptides display a proline-rich domain (N-terminal part) and a cysteine-rich domain (C-terminal part) stabilized by three conserved disulfide bonds whose arrangement has not yet been characterized. The recombinant penaeidin-3a of Litopenaeus vannamei (63 residues) and its [T8A]-Pen-3a analogue were produced in Saccharomyces cerevisiae and showed similar antimicrobial activity. The solution structure of the [T8A]-Pen-3a analogue was determined by using two-dimensional 1H NMR and simulated annealing calculations. The proline-rich domain, spanning residues 1-28 was found to be unconstrained. In contrast, the cysteine-rich domain, spanning residues 29-58, displays a well defined structure, which consists of an amphipathic helix (41-50) linked to the upstream and the downstream coils by two disulfide bonds (Cys32-Cys47 and Cys48-Cys55). These two coils are in turn linked together by the third disulfide bond (Cys36-Cys54). Such a disulfide bond packing, which is in agreement with the analysis of trypsin digests by ESI-MS, contributes to the highly hydrophobic core. Side chains of Arg45 and Arg50, which belong to the helix, and side chains of Arg37 and Arg53, which belong to the upstream and the downstream coils, are located in two opposite parts of this globular and compact structure. The environment of these positively charged residues, either by hydrophobic clusters at the surface of the cysteine-rich domain or by sequential hydrophobic residues in the unconstrained proline-rich domain, gives rise to the amphipathic character required for antimicrobial peptides. We hypothesize that the antimicrobial activity of penaeidins can be explained by a cooperative effect between the proline-rich and cysteine-rich features simultaneously present in their sequences.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12842879}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12842879 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12842879}}
==About this Structure==
==About this Structure==
-
1UEO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Litopenaeus_vannamei Litopenaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UEO OCA].
+
1UEO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Litopenaeus_vannamei Litopenaeus vannamei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UEO OCA].
==Reference==
==Reference==
Line 33: Line 37:
[[Category: Helix]]
[[Category: Helix]]
[[Category: Proline-rich]]
[[Category: Proline-rich]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:06:58 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:49:54 2008''

Revision as of 16:49, 28 July 2008

Image:1ueo.png

Template:STRUCTURE 1ueo

Solution structure of the [T8A]-Penaeidin-3

Template:ABSTRACT PUBMED 12842879

About this Structure

1UEO is a Single protein structure of sequence from Litopenaeus vannamei. Full experimental information is available from OCA.

Reference

Solution structure of the recombinant penaeidin-3, a shrimp antimicrobial peptide., Yang Y, Poncet J, Garnier J, Zatylny C, Bachere E, Aumelas A, J Biol Chem. 2003 Sep 19;278(38):36859-67. Epub 2003 Jul 3. PMID:12842879

Page seeded by OCA on Mon Jul 28 19:49:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ueo"
Personal tools