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2az1

From Proteopedia

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{{STRUCTURE_2az1| PDB=2az1 | SCENE= }}
{{STRUCTURE_2az1| PDB=2az1 | SCENE= }}
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'''Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum'''
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===Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum===
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==Overview==
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Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
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{{ABSTRACT_PUBMED_16293253}}
==About this Structure==
==About this Structure==
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[[Category: Halophilic]]
[[Category: Halophilic]]
[[Category: Surface charge]]
[[Category: Surface charge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:51:46 2008''

Revision as of 16:51, 28 July 2008

Image:2az1.png

Template:STRUCTURE 2az1

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum

Template:ABSTRACT PUBMED 16293253

About this Structure

2AZ1 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.

Reference

Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum., Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D, FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253

Page seeded by OCA on Mon Jul 28 19:51:46 2008

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