1xx0

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{{STRUCTURE_1xx0| PDB=1xx0 | SCENE= }}
{{STRUCTURE_1xx0| PDB=1xx0 | SCENE= }}
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'''Structure of the C-terminal PH domain of human pleckstrin'''
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===Structure of the C-terminal PH domain of human pleckstrin===
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==Overview==
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Pleckstrin is the major target of protein kinase C (PKC) in blood platelets. Its phosphorylation triggers responses that ultimately lead to platelet activation and blood clot formation. Pleckstrin consists of three domains: a pleckstrin homology (PH) domain at both termini and a central DEP (Dishevelled, Egl-1, Pleckstrin) domain. Here, we report the solution nuclear magnetic resonance (NMR) structure of the C-terminal PH domain (C-PH) of human pleckstrin-1. We show that this PH domain binds phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P2) with high specificity in protein lipid overlay assays. Using NMR titration experiments and mutational analysis, residues involved in binding to PtdIns(3,4)P2 are identified. The binding site is formed by a patch of basic residues from the beta1 and beta2 strands and the beta1-beta2 loop. Since PtdIns(3,4)P2 is an important signaling molecule in platelets, our data suggest a C-PH dependent regulation of pleckstrin function in response to PtdIns(3,4)P2.
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(as it appears on PubMed at http://www.pubmed.gov), where 15698571 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15698571}}
==About this Structure==
==About this Structure==
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1XX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX0 OCA].
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1XX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XX0 OCA].
==Reference==
==Reference==
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[[Category: Pleckstrin]]
[[Category: Pleckstrin]]
[[Category: Pleckstrin homology]]
[[Category: Pleckstrin homology]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:36:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:51:42 2008''

Revision as of 16:51, 28 July 2008

Image:1xx0.png

Template:STRUCTURE 1xx0

Structure of the C-terminal PH domain of human pleckstrin

Template:ABSTRACT PUBMED 15698571

About this Structure

1XX0 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure and phosphatidylinositol-(3,4)-bisphosphate binding of the C-terminal PH domain of human pleckstrin., Edlich C, Stier G, Simon B, Sattler M, Muhle-Goll C, Structure. 2005 Feb;13(2):277-86. PMID:15698571

Page seeded by OCA on Mon Jul 28 19:51:42 2008

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