2hum

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[[Image:2hum.gif|left|200px]]
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{{STRUCTURE_2hum| PDB=2hum | SCENE= }}
{{STRUCTURE_2hum| PDB=2hum | SCENE= }}
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'''Crystal structure of T4 Lysozyme D72C synthetic dimer'''
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===Crystal structure of T4 Lysozyme D72C synthetic dimer===
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==Overview==
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Previous studies of symmetry preferences in protein crystals suggest that symmetric proteins, such as homodimers, might crystallize more readily on average than asymmetric, monomeric proteins. Proteins that are naturally monomeric can be made homodimeric artificially by forming disulfide bonds between individual cysteine residues introduced by mutagenesis. Furthermore, by creating a variety of single-cysteine mutants, a series of distinct synthetic dimers can be generated for a given protein of interest, with each expected to gain advantage from its added symmetry and to exhibit a crystallization behavior distinct from the other constructs. This strategy was tested on phage T4 lysozyme, a protein whose crystallization as a monomer has been studied exhaustively. Experiments on three single-cysteine mutants, each prepared in dimeric form, yielded numerous novel crystal forms that cannot be realized by monomeric lysozyme. Six new crystal forms have been characterized. The results suggest that synthetic symmetrization may be a useful approach for enlarging the search space for crystallizing proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 17050682 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17050682}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: T4 lysozyme synthetic dimer]]
[[Category: T4 lysozyme synthetic dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:38:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:53:28 2008''

Revision as of 16:53, 28 July 2008

Image:2hum.png

Template:STRUCTURE 2hum

Crystal structure of T4 Lysozyme D72C synthetic dimer

Template:ABSTRACT PUBMED 17050682

About this Structure

2HUM is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

An approach to crystallizing proteins by synthetic symmetrization., Banatao DR, Cascio D, Crowley CS, Fleissner MR, Tienson HL, Yeates TO, Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16230-5. Epub 2006 Oct 18. PMID:17050682

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