1pvw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1pvw.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1pvw.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pvw| PDB=1pvw | SCENE= }}
{{STRUCTURE_1pvw| PDB=1pvw | SCENE= }}
-
'''3,4-dihydroxy-2-butanone 4-phosphate synthase from M. jannaschii'''
+
===3,4-dihydroxy-2-butanone 4-phosphate synthase from M. jannaschii===
-
==Overview==
+
<!--
-
Skeletal rearrangements of carbohydrates are crucial for many biosynthetic pathways. In riboflavin biosynthesis ribulose 5-phosphate is converted into 3,4-dihydroxy-2-butanone 4-phosphate while its C4 atom is released as formate in a sequence of metal-dependent reactions. Here, we present the crystal structure of Methanococcus jannaschii 3,4-dihydroxy-2-butanone 4-phosphate synthase in complex with the substrate ribulose 5-phosphate at a dimetal center presumably consisting of non-catalytic zinc and calcium ions at 1.7-A resolution. The carbonyl group (O2) and two out of three free hydroxyl groups (OH3 and OH4) of the substrate are metal-coordinated. We correlate previous mutational studies on this enzyme with the present structural results. Residues of the first coordination sphere involved in metal binding are indispensable for catalytic activity. Only Glu-185 of the second coordination sphere cannot be replaced without complete loss of activity. It contacts the C3 hydrogen atom directly and probably initiates enediol formation in concert with both metal ions to start the reaction sequence. Mechanistic similarities to Rubisco acting on the similar substrate ribulose 1,5-diphosphate in carbon dioxide fixation as well as other carbohydrate (reducto-) isomerases are discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12904291}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12904291 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12904291}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Steinbacher, S.]]
[[Category: Steinbacher, S.]]
[[Category: Riboflavin biosynthesis]]
[[Category: Riboflavin biosynthesis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:32:50 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:01:34 2008''

Revision as of 17:01, 28 July 2008

Image:1pvw.png

Template:STRUCTURE 1pvw

3,4-dihydroxy-2-butanone 4-phosphate synthase from M. jannaschii

Template:ABSTRACT PUBMED 12904291

About this Structure

1PVW is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate: implications for the catalytic mechanism., Steinbacher S, Schiffmann S, Richter G, Huber R, Bacher A, Fischer M, J Biol Chem. 2003 Oct 24;278(43):42256-65. Epub 2003 Aug 6. PMID:12904291

Page seeded by OCA on Mon Jul 28 20:01:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pvw"
Personal tools