2gi9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2gi9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2gi9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2gi9| PDB=2gi9 | SCENE= }}
{{STRUCTURE_2gi9| PDB=2gi9 | SCENE= }}
-
'''Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy'''
+
===Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy===
-
==Overview==
+
<!--
-
Structural studies of uniformly labeled proteins by magic-angle spinning NMR spectroscopy have rapidly matured in recent years. Site-specific chemical shifts of several proteins have been assigned and structures determined from 2D or 3D data sets containing internuclear distance information. Here we demonstrate the application of a complementary technique for constraining protein backbone geometry using a site-resolved 3D dipolar-shift pulse sequence. The dipolar line shapes report on the relative orientations of 1H-15N[i] to 1H-15N[i+1] dipole vectors, constraining the torsion angles phi[i] and psi[i]. In addition, from the same 3D data set, several 1H-15N[i] to1H-15N[i+2] line shapes are extracted to constrain the torsion angles phi[i], psi[i], phi[i+1], and psi[i+1]. We report results for the majority of sites in the 56-residue beta1 immunoglobulin binding domain of protein G (GB1), using 3D experiments at 600 MHz 1H frequency. Excellent agreement between the SSNMR results and a new 1.14 A crystal structure illustrate the general potential of this technique for high-resolution structural refinement of solid proteins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16522090}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16522090 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16522090}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Wylie, B J.]]
[[Category: Wylie, B J.]]
[[Category: Gb1]]
[[Category: Gb1]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:08:11 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:18:40 2008''

Revision as of 17:18, 28 July 2008

Image:2gi9.png

Template:STRUCTURE 2gi9

Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D Dipolar-Shift Solid-State NMR Spectroscopy

Template:ABSTRACT PUBMED 16522090

About this Structure

2GI9 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

Backbone conformational constraints in a microcrystalline U-15N-labeled protein by 3D dipolar-shift solid-state NMR spectroscopy., Franks WT, Wylie BJ, Stellfox SA, Rienstra CM, J Am Chem Soc. 2006 Mar 15;128(10):3154-5. PMID:16522090

Page seeded by OCA on Mon Jul 28 20:18:40 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gi9"
Personal tools